| Two parts are included in this thesis. The first part is review, and the second is research reports. The review involves the history, the basic principle, characteristics and the applications of chemiluminescence systems in pharmaceutical analysis in recent years. The research report focused on the applications of pharmaceutical analysis based on chemiluminescence. The main content is as follows:1. The binding of dipyridamole to bovine serum albumin was studied using the technique of ultracentrifuge combined with chemiluminescence analysis. In the presence of alkaline medium, dipyridamole can enhance the emission intensity of the chemiluminescence reaction of luminol-K3Fe(CN)6. The calibration curve was linear over the concentration range of 1.0×10-6 mol/L to 1.0×10-8 mol/L for dipyridamole. The dipyridamole and BSA were mixed in different concentration ratios in PH=7.4 phosphate buffer (containing 0.9% NaCl), and incubated at 37±0.5℃in an incubator for 40min. The ultracentrifuge sampler was utilized to sample free dipyridamole from mixed solution. The data obtained was analyzed with the Scatchard analysis plot. The estimated association constant (K) and the number of the binding sites (n) on one of BSA were 1.86×105 (L/mol) and 6.06, respectively.2. Qinghaosu is naturally occurring sesquiterpene endoperoxide, in the presence of hemin in alkaline medium, and qinghaosu can oxidize luminol to produce strong chemiluminescence. Coupled with liquid extraction, qinghaosu in human urine is rapidly analyzed with simple instrumentation. Under the optimum conditions, the chemiluminescence increment produced was proportional to the concentration of qinghaosu in the range of 1.0×10-6 mol/L to 1.0×10-8 mol/L (R=0.9982), with a detection limit (3σ) of 2×10-9 mol/L. At the flow rate of 1.6 mL/min, the whole process including sampling and washing could be completed in 1.5 min offering the analyzing frequency of 40 h-1 accordingly, and the relative standard deviation (RSD) was less than 1.2% (n=9). The possible mechanism of qinghaosu oxidizing luminol reaction is discussed.3. Chemiluminescence generated by ascorbic acid reacting with fluorescein-copper-surfactant could be selectively quenched in the presence of uric acid in alkaline solution. Based on the chemiluminescence quenching, a method for directly determining uric in urine with chemiluminescence detection and flow injection was developed. The calibration linear range was 0 to 10 mg/L, obeyed equationΔI=-306.58c+3557.9 (n=5, R=0.990). The relative standard deviation (RSD) was 1.2%(n=11). The proposed method presented good selectivity and sensitivity in the application to urine samples. Flow injection also promised the simplicity of the proposed method. The method was simple, selective and sensitive for determining uric acid in urine sample without pre-separation.4. In this study, we presented a method to prepare a transparent nano scale TiO2 film on glass with the sol-gel method by using Ti(OC4H9)4 as precursor, pure ethanol as solvent and hydrochloric acid as catalyst. Experimental conditions to obtain the transparent film were investigated. The films were characterized with XRD method, and the result indicated that the anatase dominated the crystal component of the TiO2 films. The photocatalyses capacity of the presented film was primarily investigated through a photocatalyses degradation of methylene blue in the presence of UV irradiation. Experiments we carried out in order to develop photocatalyses luminescence analysis.
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