Experimental Study Of Bushen Zhuanggu Prescription On Ovariectomized Rats

Objects Cytokines are essential for hematopoiesis and immune responses, and they play a key role in the defense against infection. In recent years , with the development of immunology, the role of cytokines have been increasingly concerned in the pathogenesis of postmenopausal, especially interleukin-6 ( IL-6 ) is an important proinflammatory cytokines. It has been demonstrated that IL-6 is closely related to osteoporosis by enormous clinical and experimental studies. IL-6 is regarded as a cytokine of regulating bone absorption. It has been demonstrated that Bushen Zhuanggu Prescription could effectively improve the function of ovary of ovrariectomized rats. It also can regulate the level of gonadal hormone. The change of level in gonadal hormone is a main cause which leads to osteoporosis .However, the machanism of Bushen Zhuanggu Prescription on osteoporosis have not been clearly claritied. The approach used here was based on the former fundermental research. We observed effect of different dose Bushen Zhuanggu Prescription groups on bone metabolism bood lipid cytokine and organism index. By using the immunohistochemistry technique, IL-6 was tested to study the mechanism of the Bushen Zhuanggu Prescription prophylaxis and treatment PMO. By conducting experiment, effects of different treatments were compared to see whether difference exists in order to provide theoretical evidence for clinical manifestation prophylaxis and treatment.Methods In this experiment, 48 unfertilized female rats were chosen as the experimental objects ,whose ages were all eight -month-old and weight from 242 to 318 grams. By doing oophorectomy operations on 40 rats, fake operations on 8 rats ,the rats were randomly classified into five groups according to their weights , 8 rats in each.They were ovariectomized group, nilestrial group and kidney tonifying herbs ( Bushen Zhuanggu Prescription, BSZG) in different doses. Both BSZG group and nilestrial group were conducted medicine treatments for 7 days after operation. The treatment period last for 12 weeks . After the last treatment, BMD ( Bone Mineral Density) was tested by Dural-EnergyX-ray Absorptiometry ( DEXA ) . And then serum lipid was measured after centrifugation. The effect of IL-6 on the 3rd lumbar vertebrae of ovariectomized rats was observed by immunohistochemistry after heart perfusion localization and decalcification by edetic acid (EDTA). Results1. Level of BMD of lumbar veterbra and thighbone in ovariectomized rats decreased ( P<0.01 ) BSZGP could preventing losing of bone mineral.2 TC, TG, LDL levels in Model rats increased, HDL serum decreased ( P0.01 ) , while other groups were opposite results.3 Model rats' weight of both uterus and adrenal is obviously lighter than sham operation and other groups.4 Model rats' immune celluar chemical response to IL-6 was positive, while other groups all decreased in both O.D. and positive cell percentage.Conclusion All the experimental groups had some therapeutic effect on increasing BMD,regulating serum lipid and decreasing the express of IL-6 in the axial bone. By systematic statistics , it was found that the result of nilestrial .moderate and high doses of BSZGP were the best. BSZG could be taken by postmenopausal women for long time to prevent osteoporosis which is related to PMO, it could also protect heart by regulating serum lipid.