| In recent years, extracapsular cataract extraction (ECCE) and intraocular lens (IOL) implantation play the main role in cataract surgery. Posterior capsule opacification (PCO) , the major complication after ECCE, will affect the paients' visual acuity seriously. PCO occurs in up to 50% of paients between 1 month and 5 years after surgery., and occurs almost 100% in children.Researchse showed that the formation of PCO is because breakdown of the blood-aqueous barrier after ECCE, which lead to growth factor and inflammatory medium enter capsular bag and act on residual lens epithelial cells on the anterior capsule.The fibroblast-like cells migrate and proliferate rapidly on posterior capsule and secrect a lot of collagen and extracellular matrix. Opaciflcation occurs as a result of the formation of fibrotic membranes on the posterior capsule. Opaciflcation will result in the formation of wrinkle and distortion, which lead to light scattering and visual acuity decrease seriouslyIn order to find out the mechanism of PCO formation, a lot of model forPCO in vitro had been established overseas that provided a good example to observe the process and mechanism of PCO formation ,but the report is seldom in domestic. In this research, tissue culture of lens epithelial cells on the posterior capsule to model posterior capsule opacification (PCO) will be erected, to observe the proliferation of cells and the affection of serum on model for PCO in vitro.the inhibition effects of pranoprofen on PCO were observed. Pranoprofen is a non-steroidal anti-inflammatory drug.In this research, we model pranoprofen whose concentration was like to that has metabolized for 4 hours in aqueous humor (0.23 mg/L) and observe the inhibition of pranoprofen on PCO formation.1. MethedTo spread out the bovine lens posterior capsule with the cell layer upwards on the surface of a 25ml culture flask in sterile condition. DMEM with 0 ,10% and 20% fetal calf serum 2.5ml used as medium were added respectively. The flask was set on ites end in 37 5%CO incubator. The rising water vapour protected the tissue from drying, forming a wet chamber. After 1 hour the flask was laid down,so that the medium covered the epithelium and continue to culture.The medium was changed every 3 days until the confulence of lens epithelial cells on posterior capsule. The cell coverage and confluence time on posterior capsule were recorded with graticule by inverted microscope, and the cell morphology was observed by Giemsa staining and scanning electron-microscope. The lens epithelial cells on posterior capsule were cultured in medium with pranoprofen whose concentration was like to that has metabolized for 4 hours in aqueous humor (0.23 mg/L), and then observed the difference of confluence time with control group.2. ResultThe lens epithelial cells migrate and proliferate rapidly on posterior capsule from equatorial region to the center. Within 8 to 18 days the cells became confluent. The cell coverage was rising with the increase of serum concentration, and the cell confluence time was shortening with that (P < 0.05). The lens epithelial cells on posterior capsule make a fibroblast-like change and secrect a lot of collagen and extracellular matrix. The PCO and wrinkles were formed obviously. The wrinkles presence were not dependent upon serum concentration, but increased in munber and decreased in time with that. The morphology of wrinkles by Giemsa staining and scanning electron-microscope show: the wrinkles almostly paralleled with each other., there were closely packed cells along that, and the direction of packed cells were alinged mainly at right angles to the wrinkles. In two kind of culture condition, Pranoprofen whose concentration was like to that has metabolized for 4 hours in aqueous humor (0.23 mg/L) can suppress the confluence of lens epithelial cells on posterior capsule (P < 0.01).3. Conclusion(1) The posterior capsule in tissue culture shows many of the changes seen in vivo, including rapid cells growth and fibrosis, wrinkles, extracellul...
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