| Objective: Acute pancreatitis is a usually disease of acute abdomen and about one-fifth patients can develop to severe acute pancreatitis　(SAP). SAP is a fatal surgical disease, often be in companion with multiple system organ failure (MSOF) and high morality with lung, heart and adrenal complication. There is a balance-system of inflammatory and anti-inflammatory in usual body. Adequate inflammatory reaction is benefit to body, but hyper-inflammatory reaction can lead to systemic inflammatory reaction syndrome (SIRS), seriously MSOF. Acute lung injury can be caused in 75 percent patients. The most important cause of early death in SAP is acute lung injury and ARDS. Experiment studies have demonstrated that cytokine and vasoactive mediators plays the important role during acute severe pancreatitis in recent years. The therapy of SAP with lung injury presently is mostly to interrupt progress of pancreatitis. The reports that experimental SAP animals with lung injury were treated by traditional Chinese medicine are few. The aim of this study is to investigate the serum lever of AMY, LIP, IL-6, TNF-α, MDA, ET, SOD in the red cells and PO2, PCO2 in arterial blood, to observe the pathologic changes for pancreas, lungs in SAP with lung injury rat model, and to study the influence of DY. This research was done to elucidate the reasonable mechanism of SAP with lung injury and influence of DY. Methods: 96 healthy S-D rats randomly divided into 12 groups: false SAP2h, 6h, 12h, 24h groups; SAP2h, 6h, 12h, 24h groups　(false treated groups); DY2h, 6h, 12h, 24h groups (treated with DY). model of SAP with lung injury was induced by contra- injection of 5% sodium taurocholate to pancreatic duct and gastric- leakage was finished by a conduit which was opened out of body. DY was injected into stomach through the conduit. Animals were killed and animals' blood was drawn in schedule time. Animals' lungs and pancreas were selected and adopted. Determining serum level of AMY, LIP, TNF-α, IL-6, ET, MDA, SOD in red-cell and PO2, PCO2 in arterial blood, pancreatic pathologic grades. Weigh pancreas, lungs, count their quotiety and observe their changes after treatment. Data was analyzed by statistics. Results: (1) Compared with F same time groups after operation, serum level of AMY, LIP, the amount of the ascites and pathology scores of pancreas in SAP rat models kept rise continuously. Difference is significantly, the longer the time, the more serious the case. Pancreas was observed edema, hemorrhage, necrosis spots; Lungs were observed edema, hemorrhage, inflammatory cell infiltration under light microscope and pulmonary atelectasis. The longer the time goes, the more serious the damage is. (2) Compared with F same time groups, The serum levels of TNF, IL-6, MDA, ET increased significantly in SAP groups. The longer the time goes, the higher the data is. (3) Compared with F same time groups, SOD in red-cell decreased significantly in SAP groups (except SAP2h). The longer the time goes, the lower the data is. (4) Compared with F same time groups, PO2 in arterial blood decreased significantly and PCO2 in arterial blood increased significantly in SAP groups (except SAP2h). (5) After gastric-leakage conduit injection of DY, the pathology changes of pancreas and lungs get better, the amount of the ascites, quotiety of pancreas and lung decrease significantly.　(6) Compared with SAP same time groups, The serum levels of AMY, LIP, TNF, IL-6, MDA, ET (except DY2h), PCO2 (except DY2h) in arterial blood decreased significantly in DY groups. (7) Compared with SAP same time groups, The levels of PO2 (except DY2h) in arterial blood and SOD in red-cell SOD in red-cell increased significantly in DY groups.Conclusions: (1) The method to contra-injection 5% sodium taurocholate to pancreatic duct to induce SAP with lung injury. Pancreas was observed edema, hemorrhage, necrosis spots; Lungs were observed edema, hemorrhage, inflammatory cell infiltration under light microscope and pulmonary atelectasis. The model i... |
