| Objective: Insulin resistance (IR) is not only the main pathophysioloy characteristic of type 2 diabetes mellitus (DM),but also the independent risk factor of hypertension ,coronary heart disease(CHD),obesity,dyslipidemia and atherosclerosis (AS). Hypothesis of inflammation in the pathogenesis of DM is paid attention to in recent years. And many lines of evidence had shown that inflammation plays an important role in the occurrence ,development and prognosis of DM. The relation between inflammatory factors and DM is the hot spot in the field of DM research. It has the important clinical significance to reveal the relation between them. Insulin should be fast bound to insulin receptors on the cell surface membrane, which induce a series cascade reaction to decrease blood glucose and increase the cell uptake of glucose. Therefore, the decreased number or affinity can weaken the role of insulin. The liver is one of the classic targets of insulin. So it is helpful to understand IR by observing the change of binding of insulin with it's receptor. The increased plasminogen activator inhibitor type 1(PAI-1) can accelerate the formation of AS. The level of PAI-1 has the close relation with the large vessels complication . Moreover, it relates with IR. We improved the traditional compound herbal medicine----"yu ye tang" which was used in treating "xiao ke zheng",and got a new compound herbal medicine prescription that has the efficacy of " yi qi zi yin, gu shen xiao ke, shu xue tong luo". So the aim of the study were as follows: 1 Through the determination of HOMA-IR and Insulin sensitive index (ISI), to observe the amelioration of IR. 2 Through observing changes of inflammatory factors, the number and binding capacity of insulin receptor, the expression of PAI-1 mRNA in liver to explore the effect of compound herbal medicine on IR. 3 Through the observation of effect of compound herbal medicine on IR, to develop a new prescription of traditional Chinese medicine which has the effect therapy for IR and DM . So providing a new method for the clinic to intervene IR and diabetes. Methods: Ninety healthy SD male rat, which weigh 208±20g, were provided by HeBei province experimental animal center. They were randomly divided into 6 groups and 15 rats in each group. Group 1 were the normal control group which were fed with the common feed. From group 2 to group 6 were fed with the high fat feed (which include 20% ripe porcine fat and 2% cholesterol, 0.5% cholalic salt). In the meantime, Group 3 were given with metformin. Group 4,5,6 were intervened with compound herbal medicine(yu ye tang hua cai) at various doses. Five rats were fed in each cage for 6 months.Rats were weighed and their blood samples were taken at the beginning and the end of the experiment. After an overnight fast, 4.5ml blood samples were taken from the medial canthus of eyes. Serum was stored at –20℃ until used. Liver tissue were taken and stored in liquid nitrogen for the determination of insulin receptor and PAI-I mRNA.1 Measurements of glucose and insulin: method of glucose oxidase for determining FBG and radio-immunity for FINS. HOMA-IR and insulin sensitivity index (ISI) were calculated to evaluate the resistance and sensitivity of insulin. And they were express by logarithm.2 TG ,TC ,HDL were measured by the ratio of chrom. LDL were calculated by the Friedewald (LDL-C=TC-TG/2.2)3 The measure of inflammatory factors: TNF-α,CRP, FFA respectively measured by radio-immunity, immunoprecipitation, the ratio of Cu2+ chrom.4 The determination of insulin receptor in liver cells: 4.1 the membrane of the rat liver cells were separated 4.2 measure the protein concentration by some liquor of membrane 4.3 the competitive binding of radio-ligand with insulin receptors 4.4 analysis with Scatchard5 Expression of PAI-I mRNA: Rat liver tissues mRNA was extracted by the quanidinum thiocyanate. Then PAI-1 and β-actin mRNA were respectively amplified by RT-PCR. The RT-PCR products were observed by agarose...
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