Study On The Capillary Electrophoresis Of Tumor Marker

According to World Health Report published in 2004, there were over ten millions of people who had cancer and over six millions of people who died of cancer all over the world every year. Founded early cancer could improve the proportion of existence of cancer patients. In this thesis, some researches for tumor markers such as N6-Methyladenosine, 1-methylguanosine, 7-methylguanosine, pseudouridine were studied. In section 2 and section 3, the electrochemical behavior of N6-Methyladenosine and 1-methylguanosine was investigated by cyclic voltammetry, linear sweep voltammetry, differential pulse voltammetry and bulk electrolysis with coulometry on a glassy carbon electrode. Simple, quick and sensitive method for respective detection of N6-Methyladenosine and 1-methylguanosine by differential pulse voltammetry. In the Britten-Robinson, the detection limit of N6-Methyladenosine and 1-methylguanosine were 9.8 10-8 mol/L 6.6 10-8 mol/L, respectively. The methods were applied to the the analysis of the synthetic urine and serum samples with recoveries 95.2 9-6-102.0%.In section 4, a capillary electrophoresis with amperdmetric detection was employed for the separation and determination 1-methylguanosine and guanosine. The 1-methylguanosine and guanosine can be well separated within 10 min in a 70cm length fused-silica capillary at a separation of 21kV in a 20mmol/L Plitzsch buffer (pH 8.2 Na2B4O7-H3BO3+NaCl). The proposed method was successfully applied to determine synthesis urine and bovine seruh albuth samples. The mean recoveries of 1 -methylguanosine and guanosine were 97.2%-101.0%.These four compounds 7-methylguanosine, pseudouridine, 8-azaguanine and 6-thioguanine were studied by used capillary electrophoresis in section 5. These four analystes could be successfully separated within 12min by using a 20mmol/L phosphate buffer (pH 7.4) as the carrier, 18kV as the applied voltage. The method has been employed satisfactorily for the determination of 7-methylguanosine, pseudouridine, 8-azaguanine and 6-thioguanine in the synthetic urine and serum samples with good reproducibility and high sensitivity.