The Appication Of Capillary Electrophoresis With Amperometric Detection In Chieses Medicine And Clinical Research

Capillary Electrophoresis (CE) is one of the most important separation techniques in recent twenty years. Because the theoretical plate number is greatly developed to tens of thousands and even millions, and the volume of sample injection reaches nano milliliter lever in CE analysis, it has been studied and applied to most analytical areas. However, the small sample injection volume and very thin capillary bring about difficulties to detection. The common methods of detection are ultra-violet (UV), laser induced fluorescence (LIF), mass spectra (MS), etc. Every method has some advantages and suffers from some drawbacks too. For example, LIF is very sensitive but the instrumentations are very expensive, and some complicated derivation procedures are often needed. Electrochemical detection is more sensitive than UV detection, and has many advantages when it combines with CE, such as simple instrumentation and operation, low cost, wide linear range, etc. So the method of CE-ED is extensively studied and applied in most analytical fields for its above advantages.The goal of this thesis is to explore some applications in pharmaceutical analysis, biochemical analysis and food analysis by CE-ED. The contents of this thesis include six chapters:1. PrefaceThe characteristics of CE, the separation models, the basic theories of CE, the CE detectors, the studies and applications of CE-ED are introduced simply. The goal and significance of this thesis are introduced too.2. Determination of six flavones in Leonurus japonicus and Leonurus japonicus Granules by Capillary Electrophoresis with Electrochemical DetectionA simultaneous determination of six flavones, including phloridzin, hesperedin, rutin, kaemperol, apigenin, quercatin, in Leonurus japonicus and three kinds of commercial Leonurus japonicus Granules by Capillary Electrophoresis with...