An Experimental Study On Bone Regeneration Induced By Bone Active Polypeptide, Coralline, Sanguisorba Officinalis L

Objective: To evaluate the effect on the frontal bone regeneration of the mice induced by Bone Active Polypeptide (BAP), to study the mechanism of bone regeneration induced by BAP. Methods: 72 Kun Ming mice were divided into 3 groups randomly, one was experimental group , the two were control groups, each was respectively 24. The mice were operated . Bone defects of 3 mm . 2 mm . 1mm in site were created on frontal bone with the use of local anesthesia, and then the defects had been filled with BAP composite Coralline in the experimental group; only Coralline in the control group A; Coralline composite Sanguisorba Officinalis L (SO) in the control group B. Postoperatively, the mice were killed at intervals of 7, 28 and 90 days, the specimens were examined histopathologically by light microscope and transmission electron microscope. Osteoblasts were counted and studied quantitatively by analysis of variance(ANOVA) to compare each group. Results: 7 days of postoperation, it was revealled that the bone defects were infiltrated by the white blood cells, degeneration and necrosis in all groups; after 28 days, the inflammation was absorbed, fibroblasts and new fine capillaries were developing actively, new bone formation were also found in the experimental group; the inflammation was absorbed in the control group A, and in the control group B, fibroblasts and many new capillaries were found. After 90 days, ossification was obvious in the experimental group, but not in the control groups. Osteoblasts were counted, P < 0.01, statistical analysis showed difference between the experimental and control groups. It was also proved by TEM. Conclusions: The frontal bone regeneration in the mice can be induced by BAP, and BAP composite Coralline was more remarkable. The mechanism of bone regeneration induced by BAP is that BAP is a variety of growth factors.