| The detection of Epstein-Barr virus and its cooperation with hepatitis Cviruses in the Hepatocellular carcinomaBackground: Hepatocellular carcinoma (HCC) remains the fifth most common cancers worldwide and severely damages human health. About five hundred thousand people died of HCC every year in the world. The etiology of HCC is still unclear. According to the epidemiological study in the high incidence rate areas, the etiology may relate to viral hepatitis, aflatoxin, water pollution, genetic factor and so on. Experts at home and abroad pay close attention to the virus agents.Virus has been associated with human malignancies. The cause of HCC is closely related to viral hepatitis. In recent years, some investigations suggested that EBV might play a role in HCC. It is confirmed by experiment that EBV can accelerate the duplication of HCV in the carcinogenesis of HCC. The former study in our experimental group suggested that EBV act in carcinogenesis and development of HCC, but EBV and HBV couldn't cooperate with each other during the course of development of HCC. In this study we detected EBV and HCV in the HCC specimens from this locality, and discussed the role and its cooperation with HCV in the carcinogenesis of HCC. PartObjective: EBV as herpesvirus with high infectious rate in the crowd has been associated with human malignancies. In recent years, some investigations suggest that EBV may play a role in HCC. In this study we detected EBV in the HCC specimens from this locality, then we discussed the role of EBV in HCC.c Methods: Polymerase chain reaction (PCR) method was processed to detect EBV BamH I W DNA and/or EBV LMP1 DNA in formalin-fixed, paraffin-embedded liver tissue speciments from hepatocellular carcinoma (HCC, 127 cases) patients. 41 liver tissue specimens from patients with calculus of intrahepatic duct served as controls. Immunohistochemistry method was also used to detect Anti-Epstein-Barr Virus LMP and Epstein-Barr Virus/LMP Ab-1 in all specimens. All the specimens were from chronic Hepatitis B patients. (Because all the specimens were from the patients with chronic Hepatitis B disease, we didn't have to consider about the discordance of HBV infection) Results: The positive ratio of EBV DNA (BAMHI W and/or LMP1) is 38.6% (49 of 127) in HCC group and 19.5% in control group (8 of 41). The positive ratios were compared between the two groups by means of the chi-square test, X2=5.028, P0.05 was considered a significant difference. Conclusions: EBV was more frequently observed in HCC group than the control group (P<0.05). The results indicate that HCC might be associated with EBV infection in this locality. Further research about the role of EBV in the carcinogenesis of HCC should bemade.Key words: Hepatocellular carcinoma; EB virusPartObjective: The importance of hepatitis B virus (HBV) and hepatitis C virus (HCV) infection in the carcinogenesis of HCC has been well established. In recent years, some investigations confirmed that EBV can accelerate the duplication of HCV in vitro experiments and suggested that EBV relate to HCC. In this study, we detect the EBV and HCV in formalin-fixed, paraffin-embedded liver tissue specimens from hepatocellular carcinoma (HCC) patients with chronic Hepatitis B disease, and discussed the role and cooperation of EBV and HCV in the carcinogenesis and development of HCC. Methods: Polymerase chain reaction (PCR) method was processed to detect EBV BamH I W DNA and/or EBV LMP1 DNA. Reverse transcriptase Polymerase chain reaction (RT-PCR) method was processed to detect HCV NS3 RNA, Immunohistochemistry Two-step method was used to detect the EBV and HCV in formalin-fixed, paraffin-embedded liver tissue specimens from hepatocellular carcinoma (HCC, 127 cases) patients. 41 specimens from patients with calculus of intrahepatic duct served as controls. All the specimens were from chronic Hepatitis B patients. (Because all the specimens were from the patients with chronic Hepatitis B disease, wedidn't have to consider about the discordance of HBV infectio...
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