Significance Of P38MAPK In Acute Lung Injury During Severe Acute Pancreatitis And Study Of The Treatment By Qing Yi Tang Decoction

Objective: To investigate the potential role of P38MAPK activation in acute lung injury induced by severe acute pancreatitis and also to observe inflammatory cytokines and endotoxin in pulmonary tissue and blood. Chinese medicine QingYiTang Decoction was used and the results may give us some new ideas for curing acute lung injury.Methods: The rats were divided into four groups:Sham operated controls (n=16); SAP model group (n=16); QingYiTang Decoction treated group (n=16); Inhibitor treated group (n=16). 1.5% sodium deoxycholate was injected inversely into the common biliopancreatic duct of rats to improve the model of SAP. All the rats were killed at 24 hours after operation. Pathological examination and some indexes of ALI were measured.Pulmonary P38MAPK and ICAM-1 activity were determined by Western Blot. And we also measured the level of NO,TNF-а,Endotoxin,Amylase, et al.Results: The levels of P38MAPK and ICAM-1 in pulmonary of model group were significantly higher than those of sham operated control group (p<0.05). Some other indexes of ALI showed obvious pathogenic changes in model group and so did the pathogenic examination of lung tissue. QingYiTang Decoction could down-regulate the expression of the P38MAPK and ICAM-1 activity. The decline of NO,TNF-а in QingYiTang Decoction group was more significant (p<0.05).Conclusions: During acute lung injury induced by severe acute pancreatitis, many factors have very important effects, such as P38MAPK activity which stimulates the activity of NF-kB, and start up the volume release of NO and TNF-а, and increase the activity of ICAM-1 obviously. QYT decoction could exert its protecting effects through eliminating P38MAPK activity, alleviating the level of NO,TNF-а, eliminating ICAM-1 activity and decreasing the higher level of endotoxin and amylase,then alleviating acute pulmonary injury. In a word, traditional and western medicine make very important effects in acute lung injury induced by severe acute pancreatitis. If applying traditional and western medicine reasonably, we will find more useful methods to prevent and cure acute lung injury induced by severe acute pancreatitis in clinic.