| Senescence is a degenerate process of tissues and organs in function and morphology in a span life, and the senescence of central nervous system has a dominant status in aging. Changes of pathology were discovered mostly in cerebral cortex and hippocampus. Liver is an important metabolic organ and its cells can produce many substance which enter into blood directly. The number of liver cells will decrease quickly when human reaches 80 years old , and a larger amount of lipofuscin are discoved in liver cells. So that it is very important to study the senescence of liver, cerebrum and hippocampus in order to learn the process and mechanism of senescence.The free radical theory of aging indicated that cellular degeneration was resulted from the excessive free radical that produced in the process of normal cellular metabolism as growth. The main reason of senescence with D-galactose is oxides and peroxides of the excessive superoxidative anion produced in the metabolism .Although anti-aging drugs have been studied for decades, much interesting was focused on the anti-aging food, especially fruits and vegetables. Carrot and tomato are common edible vegetables for people and in which there are many carotenoids such as P-carrotene, lycopene, cryptoxanthin, lutein, and zeaxanthin. P-carrotene and lycopene are considered to possessing antioxidant properties of carotenoids and they can quench singlet oxygen and trap peroxyl radicals, and cryptoxanthin, lutein, and zeaxanthin have potentially antioxidant ability as well. These carotenoids can be absorbed preferably by body within fat condition. The concentration of P-carrotene and lycopene in blood plasma and tissues will be improved by increasing consumption of these vegetables, and then the antioxidant capacity of body would be improved . Therefore, the carotenoids can make body prevent from early aging and age-related diseases by inhibiting produce of free radicals. It was worth indicating that the capability of cleaning up free radicals and the effect of antioxidation of vegetables and fruits are the complex results of all the nutrients, but not depending on one or several ingredients.In our studies, methods of morphology and function were used in order to search for the effects of carrot and tomato on liver,cerebral cortex and hippocampus of the central nervous system of the Wistar rats. Our results could provide some theoretics basis for developing foods and their produces which rich in carotenoids. The 6-week-old male rats were divided into four groups at random, ten in each group. Normal control group was given saline(100ml/kg) and edible oil(0.75ml each rat) every day, D-Gal group was given D-galactose(100mg/kg) and edible oil (0.75ml each rat) every day, Carrot group was given D-galactose(100mg/kg) and carrot (15g each rat ,contenting edible oil 0.75ml) every day, Tomato group was given D-galactose (100mg/kg) and tomato (15g each rat, contenting edible oil 0.75ml) every day. Saline water and D-galactose were given by subcutaneous injection of neck, edible oil, carrot and tomato were fed with feedstuff. Saline water and D-galactose were given for 25 weeks. After one week stopped the injection, we collected inferior vena cava blood, liver, cerebral cortex and hippocampus, then the contents of MDA in serum and tissues, and the activities of SOD and GSH-Px in blood and tissues were measured with biochemistry methods. Common morphology and ultrastructures of tissues were observed with light microscope and electron microscope.The results showed that MDA contents of normal control group, carrot group and tomato group decreased more significantly than that in D-Gal group, and the activity of SOD and GSH-Px increased more significantly than that in D-Gal group. Under light microscope, large amounts of liver cells and neurons in normal control group, a large of extensive putrescenced liver cells and condensed, vacuolated neurons in D-Gal group, and most liver cells were not changed and only small neurons slightly vacuolated in carrot group and in tomato group were obs... |
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