| Objective: To investigate the expression of Epidermal Growth Factor (EGF) and Epidermal Growth Factor Receptor (EGFR) in operative cavity after functional endoscopic sinus surgery, and to examine their roles during the progression of wound healing after functional endoscopic sinus surgery. In order to provide theoretica basis for the mechanism of wound healing of operative cavity.Materials and Methods: During October 2002 to May 2003, 10 cases of type I and 10 cases of type II of chronic sinusitis and polyps were enrolled (All patiens who had been operated on by FESS at the Department of otorhinolarygology at XiNan Hospital). The study was designed as a single center prospective pilot study. The samples were taken from 20 patients at four diffrent stages, including Preoperative stage, 1-2-week, 4-6-week, and 10-12-week after FESS and compared to 10 controls. Identification of EGF and EGFR was sought by using immunohistochemmistry, Western blotting and reverse transcriptase-polymerase chain reaction (RT-PCR).Result:1. Mass morphological observation: Nasal and sinus cavity got clean within 1 to 2 weeks postoperatively. Operative cavity had the De-mucous reaction from 4 to 6 weeks, such as mucous edema, vesicles, granulation tissue, mini-polyps, fibrous hyperplasia, and adhesion or regenerated diseases may appear in this stage. In 10-12-week group, nasal and sinuous cavity is brought to stage of complete epithelization.2. (1) Immunohistochemical analysis of tissue sections from control revealed intense membrane staining for the EGF within the respiratory nasal epithelium, which was predomominantly localized at the level of the columnar epithelial layers and weakly staining was found in some submucosal gland. Similar staining patterns were observed for EGFR. (2) In chronic sinusitis and polyps, Immunoreactivity for both EGF and EGFR was found in the epithelial cells and inflammatory cells and in some submucosal gland cells and basementmemberane. There was stronger staining of EGFR and EGF in this group than in controls. (3) In 1-2-week group, staining of both EGF and EGFR was found in inflammatory cells and epithelial cells of operative cavity. (4) In 4-6-week group, Immunoreactivity for both EGF and EGFR was found in the epithelial cells and inflammatory cells and some submucosal gland cells, and in basement memberane and vascular endothelial cells. (5) In 10-12-week group, staining of both EGF and EGFR was found in the columnar epithelial cells, weakly staining was found in some submucosal gland.3. Immunohistochemical and Western blotting results showed: In 1- 2-week group after surgery, compared with control group, a significant decrease of secretion of EGF and EGFR protein was found (P<0.05). Compared with control group, in 4-6-week group after surgery and preoperative group, the significant increase of expression of EGF and EGFR protein was found (P0.01). In 10- 12-weeks group after surgery, the secretion of EGF and EGFR protein tended to the level of control group, and no significant difference was found (p>0.05). In S group and in P group respectively, the significant difference was found between each others, but no significant difference was found beween S group and P group at the same stage.4. RT-PCR result showed: Compared with control group, in 1-2-week group after surgery, the significant decrease of expression of EGF mRNA and EGFR mRNA was found (p<0.05). 4-6-week group after surgery and preoperative group showed significant increased (p0.01). However, in 10-12-week group after surgery, compared with control group, no significant change was found (p>0.05). In S group and in P group respectively, the significant difference was found between each others, but no significant difference was found beween S group and P group at the same stage. This result suggests that expression of EGF mRNA and EGFR mRNA was in accord with expression of EGF and EGFR protein.Conclusion:1 .Expression of EGF and EGFR in normal nasal cavity mucosa suggested that they played an important role in maintenance no...
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