| The peripheral nerve injury is always accompanied by Wallerian degeneration in the wound and distal regions of the nerve and apoptosis of some related neurons.But some harmful factors such as wrong growth each other of motor and sensory neuritis in regeneration regions, the fibroplasias in nerve gap and atrophy of axon terminals after a long time of denervation always result the nerval function recovery is always dissatisfactory.So, it has become focus in clinical and basic research to investigate morphological changes of the injured nerve and measures of promoting peripheral nerve regeneration.Objective 1) To study the effects of different extent of the wallerian degeneration on injured sciatic nerve regeneration and recovery of neural function in rat .2) To investigate the mechanisms and effects of Interleukin-1 beta on nerve regeneration.3) To research morphological changes of degenerating and regenerating sciatic nerve following injury, and grope for medical image visualization methods for peripheral nerve regeneration.The findings of these researches may provide theoretical basis for clinical therapy to peripheral nerve injury.MethodsIn the part one of the research, the rats' sciatic nerve was transected and then bridge-connected with silicone tube, The wallerian degeneration of transected sciatic nerve were intervened with recombinative human Interleukin-1 beta(IL-1β) and dichloromethylene diphosphonate-containing liposome(Cl2MDP) in respectively. Macrophage congregation in the local and the extent of wallerian degeneration were observed by pathological and immunohistochemical means on the 1,3,5,7,15,30,60,90 days after injury, and the outcome of sciatic nerve function index(SFI)and Electrophysiolgical assessmentwere observed, by which the effects of different extent of wallerian degeneration on the nerve regeneration was evaluated.In the part two of the research, the dynamic expression of the nerve growth factor (NGF)and Glial cell line-derived neurotrophic factor(GDNF) in the local were inspected with immunohistochemical technique, associating with pathology and morphometric analysis, the mechanisms and effects of Interleukin-1 beta were explored on injured peripheral nerve in rats. In the part three of the research, The images of serial slices which were stained with trichrome stain was taken by micro-photograph system and inputted into computer by which the image matching and 2-D integrate processing were completed.The 3-D reconstruction and display of typical morphological structure of the injured nerve was accomplished in SGI workstation, and then we can explore morphological character of sciatic nerve regeneration and quantization method for nerve regeneration process.Result1.Soft red crisp colloid was seen in the silicone tube for 3 and 7 days after injury.15 days later it was observed that a soft red strip has connected two sides of transected sciatic nerve in the IL-1β group.After 30 to 90 days all of the newborn nerve became thicker , more tenacious, and its color became brighter than before, whereas, the diameter of the rebirth sciatic nerve in the IL-1β group were bigger than that of Cl2MDP and control groups.But the difference between the Cl2MDP group and control group has been inapparent .2.The findings in the light microscope and electron microscope indicated that the time of macrophage assemble in the local region of injury and the time of axon rupture and myelin collapse in the IL-1β group was earlier than that of other groups, and the cleanness of degenerative tissue was more faster in the early stage in the IL-1β group.There was fewer hyperplastic collagen fiber in the IL-1β group.The indexes of regeneration nerve were superior to that of control group.On the contrary, there is fewer macrophage which invade into the local region in the early stage in the Cl2MDP group, and the elimination of degenerative tissue was also slow.The distributing density of the newborn nerve also was inferior. 3.The recovery rate of sciatic nerve functional inde...
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