Differential Proteome Analysis Of Peripheral Sensory And Motor Nerve Before And After Wallerian Degeneration By ITRAQ

Objective:To screen high specific differential proteins expression in Wallerian degenerated sensory and motor nerves, established the sensory and motor nerve injury model, aiming to make the foundation of the progressive mechanism research of peripheral nerve chemotactic regeneration.Methods:A total of 60 adult male wistar rats were chosen as our research subjects and divide them into 3 groups randomly. A (n=20) and B (n=20) groups were performed to establish the femoral nerve transection model (A) and nerve root injury model (B) respectively, and the other group C (n=20) is normal control groups of group B. Group A choose the contralateral normal nerve as control. The muscle branch of femoral nerve and the ventral nerve roots are selected as the motor fibers, while the saphenous nerve and dorsal nerve roots are selected as sensory fibers. We harvested the degenerated nerves after 7 days and carried out the iTRAQ coupled with 2D LC-MS/MS to mark the isotope, analyze the mass spectrometry and detect differential proteins. Then, we use DAVID to deal with the differential proteins for further screening the protein associated with the specific regeneration machanism of peripheral nerve.Results:(1) In the femoral nerve transection model, a total of 3358 differential proteins were identified. Compared with the control group, there are 293 and 417 proteins identified respectively in the damaged muscular branches of the femoral nerve and saphenous nerve. By further intersection analysis,107 specific differential proteins were identified in damaged muscular branches of the femoral nerve, while 231 specific differential proteins were identified in damaged saphenous nerve.(2) In the nerve root injury model, a total of 4312 differential proteins were identified. Compared with the control group, there are 637 and 626 proteins identified respectively in the damaged ventral nerve root and dorsal nerve root. By further intersection analysis,195 specific differential proteins were identified in damaged ventral nerve root, while 184 specific differential proteins were identified in damaged dorsal nerve root.Conclusion:Through the technique of iTRAQ, we can detect the differential proteins and screen the specific proteins in the damaged sensory and motor nerve, which may lay the foundation of the progressive mechanism research of peripheral nerve chemotactic growth.