Studies On The Bioactive Constituents Of Secondary Metabolites Of Marine-Derived Actinomycetes Z3001

We have undertaken the screening for novel cell cycle inhibitors and apoptosis inducers from the marine-derived actinomycetes, Z3001 by use of a mouse cdc2 mutant cell line, tsFT210. In anticipation of achieving the above objectives, marine-derived actinomycetes, Z3001 have been screened by using this model, the EtOAC extracts of strain Z3001 showed bioactivities of inhibiting the cell cycle progression and cytoclasis of tsF210 cells. Thus, we selected them for further investigation.Eleven compounds were isolated from the bioactive part, EtOAC extract of strain Z3001, by repeated column chromatography on Sephadex LH-20, silica gel and RP18, followed by reverse-phase high performance liquid chromatragraphy. The structures of compounds were elucidated mainly by use of spectroscopic method (UV, IR, MS, 1D-NMR, 2D-NMR) and according to their physicochemical properties: palmitic acid(l), 2,3-dihydroxypropyl palmitate (2), 3,6-diene-2,8(8-hydroxy-9-methyl) decyl-l,5-dioxocane(4), 3, 4-Dihydroxy-2-methylpyridine (5), cyclo-(Val-Pro)(6), cycle- (Pro-Leu) (7), 4- (2-hydroxyethyl)phenol(8), 1H-indole-3-carboxylic acid(9a), lH-indole-3-carboxamide (9b), 2-(6-hydroxy-9H-purin-9-yl) -5- (hydroxymethyl) -tetrahydrofuran- 3,4-diol( 1 0). The structure of compound ( 3 ) is still in affirming.Bioassay results indicated that (1)(2)(4) show cell cycle inhibition and cytoclasis of tsFT210 cells. Compound (4) showed a strong apoptosis and cytoclasis of tsFT210 cells. Compound (1)(4)(10) show cell cycle inhibition and cytoclasis of tsFT210 cells, the were the main antitumor active compounds of EtOAC extract of strain Z3001.Bioassay results indicated that we isolated the main antitumor active compounds of strainZ3001 successfully which showed that this antitumor model and the method were exact and available in achieving bioactive components from marine microbes.