Study On The Active Constituents In Fermentation Broth Of Two Strains Of Marine Actinomycetes

This thesis has studied on the bioactive-guided isolation and structural determination of active constituents in fermentation broth from two strains of marine actinomycetes No. Ms252 and No.172221 that respectively showed antifungal effect and synergistic antifungal effect and cytotoxic activity for HCT-116 in early screening. Fifteen compounds were isolated from the active fractions by silica gel column, ODS open column chromatography, preparative HPLC and other chromatographic techniques. Their structures were identified as 3,3-bis(3'-indolyl)-1,2-propanediol (1), staurosporine aglycon (2),5-(9H-pyrido[3,4-b]indol-1-yl)-2-furanmethanol (3), 1-[5-(hydroxymethyl)-2-furanyl]-9H-Pyrido[3,4-b]inddole-3-carboxylic acid (4), methylcholestane-3β,5α,6β-triol (5), ergostane-β-D-glucopyranoside (6),β-daucosterol (7), cyclo-(Pro-Leu) (8), cyclo-(Pro-Val) (9), proline (10), palmitic acid (11), N-(phenylmethyl)-carbamic acid(12),1,3-propanediyl benzeneacetic acid ester (13), daidzein (14) and adenosine (15) by modern spectroscopic methods (MS, 1D and 2D-NMR). Among them, compounds 1～4 are indole alkaloids, compounds 5～7 are steroids, compounds 8～9 are cyclic dipeptides usually seen in the microorganism metabolites, whereas 1,3～4 and 6 was isolated from actinomycete for the first time. Compounds 12～13 are new natural products. Then these compounds were evaluated on the activity through co-operations. We found that compounds 1～4 showed certain degree antifungal activity but no significant synergistic effect, compounds 5～15 showed weak cytotoxic activity for HCT-116.