A Experimental Study Of The Effect Of Yue-hua Capsule To Cytokine TH1/TH2 In Rats With Multi-drug Resistant Tuberculosis (MDR-TB)

Objective: (1)To observe the therapeutic efficacy of Yue-hua Capsule to rats with MDR-TB. (2)To assess the mechanism of action of the regulation of Yue-hua Capsule to cytokine TH1/TH2 by molecular immunology.(3)To comprehend the effect of yue-hua Capsule to the injury of liver and kidney by antituberculosis chemotherapeutics.Method: Build the infection model by injection Multi-drug resistant Bacillus tuberculosis (normal saline)into the vein of tail of Kunming genera rats and then group them into 4 groups random(Build the blank group). Drench them corresponding drugs 28 days continuously(Drench NS to the blank group , the model group).(1)To observe the change of weight and temperature, (2) To observe the change of lung organ exponent, thymus organ exponent, lung affection exponent, color, excreta, tubercler, the composition that macrophage phagocytize tubercler, etc.(3)To observe the change of CD4+CD8+ in blood by immunohistochemistry staining method and To observe the change of IFN- y , TNF- a , IL-2, IL-10 in serum by ELISA assay. By pathological section, observing the effect of yue-hua Capsule to the injury to liver and kidney by antituberculosis chemotherapeutics drug.Result: To campare with model group: the latter line antituberculisis grug group, Yue-hua Capsule group and the Yue-hua Capsule&the latter line antituberculosis drug group all could make multi-drug resistant-tuberculosis rats' body temperature descent, and make those body weigh increase (P<0.01 ~ 0.05 ) . And they could evidently descent the lung pathologic change exponent, lung organ exponent, necrotic tubercler and macroohage pathagocytize tubercler(p<0.01). They could improve the thymus organ exponent, increase the number of CD4I\ CDS in T cell subpopulation, make the content of IFN- Y TNF- a > IL-2 belonging to Thl cell factor increase, and make the content of IL-10 belonging to Th2 cell factor discrease(p<0.01). To compare with respective therapying group: There was no significant different in the therapeutic efficacy between Yue-hua Capsule group and the latter line antituberculosis drug group(p>0.05); Yue-hua Capsule&the latter line antituberculosis drug group had better efficacy than Yue-hua Capsule group and the latter line antituberculosis drug group ( P<0.01 ~ 0.05 ) . (3)To compare with the latter line antituberculosisgroup ( P<0.01 ~ 0.05 ) . To compare with the latter line antituberculosis drug group: to observe by the result of pathological histology, liver multiple thanatosis and glomerulus lesion had lessened in Yue-hua Capsule group and the latter line antituberculosis drug group.Conclusion: (Din the animal experiment, we can get good therapeutic efficacy from using Yue-hua Capsule to treat MDR-TB. Yue-hua Capsule&the latter line antituberculosis drug made the effect enhance on therapying the Multi-drug resistam iberculosis rats model. (3)Yue-hua Capsule could kill Bacillus tuberculosis in body by elevate the immune function of body, increase the number of T cell subpopulation, promote the secretion of IFN- Y and IL-2 belonging to Thl cell factor, regulate TNF-a to the certain range, discrease the content of IL-10, promote Th2 cell's transformation to Thl cell, form the scrofulous gumma to prevent the defusing of Bacillus tuberculosis. Yue-hua Capsule had the certain protecting effect on liver and kidney pathologic lesion induced by antiphtisy and chemotherapy.