Effect Of Enteral Econutrtion On Gut Barrir, Serum TNF, IL-6 And Endotoxin In Serious Intra-abdominal Of Rats

The nourishment support of the surgical operation patient has become the importance parts of the modern surgical operation treatment, being seen as important advanced of medical science in the twenty centuries. The nutriment of EN has the main parts of benefits in the intestine, such as promoting the protein synthesizes, enhancing the immunity function, alleviating the damage of the stress response, promoting the releases of intestine hormone and immune globulin, improving intestinal microcirculation and reducing the severity of intestinal muscosal injury. Some investigation indicated that total calories 20% of enteric nutrition could avoid the injury of gut barrier faction and intestinal bacterial translocation. The function of medicine is more weight than its nutrition in some serious infection patients. The aim of the study was to investigate the effect of enteral ecoimmunonutrtion on gut barrir, serum TNF, IL-6 and endotoxin in serious intra-abdominal of the rats In this experiment, we use 40 SD rats that are divided into four groups: (1) artificial operation groups (n=10), (2) cecal ligation and puntures (CLP) groups (n=10), Enteric Nutrition (EN) groups (n=10), (4) Econonutriyion groups (n=10). The suspension was exposed to the environment for two hours in this experiment, all animals were killed after five days and were examined the tissue of intestine, at the same time, examined the serum TNF, IL-6 and endotoxin in different time during the study. Passing optical microscope observe, we found: The intestine muscoma instruction of artificial operation groups big rats had few changing. Others had different injury, especially the CLP groups were serious. The intestine muscoma instruction of EN groups was not serious compared with the CLP groups. The level of the serum TNF, IL-6, endotoxin is start going up after 6 hours the model being made. The serum TNF level of artificial operation and CLP groups goes up obviously during 24 hours, and the 48 hours reach the high peak, and then having the descent slightly The serum TNF level of the enteric nutrition (EN) groups is lower than that of the CLP groups obviously, but still higher than that of artificial operation groups; Each hour of the Econonutriyion groups orders mutually as well all lower than the set of EN. (p<0.01). Our study show that the Econonutriyion can cut down the level of the serum TNF, IL-6, endotoxin, moderate the poisonous material in an inside intestine, adjust intestine for tiny ecosystem, ameliorate intestine inside environment and lowering the infection rate and death rate.