The Expression Of Matrix Metalloproteinases-2 And It'stissue Inhibitor-2 And Microvessel Ddensity In Endometrial Carcinoma

Objective carcinoma of endometrium is one of three malignant tumor in women reproduction system , in the recent twenty years, the incidence rate of endometrial carcinoma which affect the women health severly have a rising trend throughout the world . But until now we have no precise knowledge of the mechanism how endometrial carcinoma happened .Study indicated that the excess expression of MMPs and the expression maladjustment between MMPs and TIMPs have close relation with the tumor's invasion and metastasis in endometrial carcinoma .The invasion to close constitution and the metastasis to distant place organs of tumor cells were complex progress. In this progress tumor cells must have the function of degrading extracelluar matrix(ECM) and basement membrane(BM) firstly.The degradation of extracelluar matrix is key approach to tumor's invasion and metastasis. This degradation function is preformed by proteinase . MMPs is an important kind of proteinase .They degraded extracelluar matrix and basement membrane and reinforced angiogenesis of tumor cells which promote tumor cells' invasion and metastasis. Many study have approved that MMP-2, MMP-9 and TIMP-1,TIMP-2 play an important role in this process. Neoplasm must form new blood vessel network in it's invasion and metastasis. Endothelial cell's circulating and blood vessel struture's forming need degradation and reconstrution of ECM .In this process MMPs played an important role . MMPs is one of accelerate factor to angiogenesis but TIMPs is one of inhibitive factor . Some study indicated that the quantity of tumor's blood vessel was relate with tumor's invasion and metastasis. MMPs accelerate tumor's invasion and metastasis through stimulating blood vessel growth .But TIMPs restrain new angiogenesis through lowering the function of MMPs in many taches. Meanwhile TIMPs also restrain the release of promote-angiogenesis factor in the Base and prevent degrading of ECM. The purpose of this study was to discuss the expression of matrix metalloproteinases-2 and it's tissue inhibitor-2 and the density of microvessel in human endometrial carcinoma tissue and its relation with the invasion and metastasis of endometrial carcinoma and provide themry evidence for the anti-tumor therapy. Method To detect the expression of MMP-2,TIMP-2,MVD immunohistochemistry were used in endometrial carcinoma tissue of 33 patients and control group composed of 8 normal endometrial samples. All spacies come from the first and the second hospital of jilin university (2002-2003) and were confirmed by pathology and which weren't hormone therapy before sugery in three month . According to standard of FIGO , the histology type of all the cases were endometrioid adenocarcinoma and included different pathology degress and surgical pathologic staging. There are 17 cases have lymph node metastasis. Result's standard The positive expression of MMP-2,TIMP-2 show brown particles in cytoplasm. According to the precent of positive cell and degree of stain were divided four level : negative (-) , slight positive (+) , moderate positive (++) , severe positive (+++).The positive expression of MVD show brown particles in blood vessel endothelial cell .A single endothelial cell was also counted no matter what lumen of blood vessel had formed or not .we choice four different sight (×200) counting the quantity of positive stain and the average quantity of blood vessel.This was micro vascular density(MVD) (x- ±s).Results The MMP-2 and TIMP-2 and MVD protein express in both normal endometrial samples and endometrial carcinoma. The positive expression proportions of MMP-2 and TIMP-2 and MVD protein in endometrial carcinoma tissue were higher than normal endometrial samples.(p<0.05). Semiquantitative analysis revealed MMP-2 staining scores in tumor cells were significantly associated with the presence of mayometrial invasion and surgical pathologic staging and lymph node metastasis (p<0.05),while TIMP-2 did not correlate with these factors. MV...