| Medicolegal pharmaceutical analysis is consultation, quantitative and qualitative analysis of doubtable material in laws and regulations as a branch of forensic medcine, which contains separation and purification of intoxicants and their product of metabolism from tissue, body fluid or excreta, and quantitative and qualitative analysis to the prototype or metabolic product of intoxicants or drugs. The most common sample in use is blood and the routine methods were reported widely, but there are seldom reports from the samples treated by formalin. If a series of pharmaceutical analysis methods on samples treated by formalin are established, it will no doubt be better for us to clarify the case and provide the proof for the trial. Pharmaceutical analysis on bio-material belongs to trace analysis because it is a complex metabolic process for intoxicants or drugs in bodies and it is also affected by formalin fixation. It is very important for us to separate, extract and purify the drugs or intoxicants themselves and their metabolite from the samples treated by formalin and then analyze them qualitatively and quantitatively. When a method or technique is selected, it should have high selectivity, separation efficiency, detection limit and veracity. The keys are its applicability, feasibility, creditability and value in medicolegal practice. In this study, a useful method was established for detecting the trace rudimental amount drugs or intoxicants in formalin fixed tissue. This technique has both accurate and practical features, and if it is applied to the medicolegal practice., it will be good to it .ObjectTo establish a method for detecting the trace rudimental amount drugs or intoxicants in formalin fixed tissue and provide to convincing proof for the medicolegal practice. MethodsThe method was determined according to the physicochemical property of the drugs or intoxicants and their metabolic process in organism.1. For the formalin fixed viscera of Guinea pig in experimental group, perchloric acid was applied in order to remove the matrix protein, methanol was applied in order to abstract the methomyl ingredient, and solid phase column extractor was applied to purify the ingredients so that it can be detected. In this procedure, Aglient 1100 Pickering 5200 high efficiency liquid phase chromatographic analysis system was applied, the detecting wave is 235nm, ZORBAX Eclipse XDB C-18 chromatographic column (4.6mm×50mm 5) was selected. For mobile phase, the solution of methylcyanide:methanol:distilled water (5:45:50) was used, its flow rate is 1.2ml/min and sample size is 10l in room temperature.2. Guinea pig lidocaine-intoxicated model was established,then killed the animals and fresh blood and viscera samples and 10% methanal antisepticise treated viscera samples were obtained. All samples were dripped 10% fast sodium sulfite before extracted by chloroform in alkalescence qualification and HPLC analysis. The equipment of HPLC is Agilent 1100, the chromatographic column is ZORBAX 250×46mm OPS, the mobile phase is alcohol phosphoric acid buffer (pH 3.5): methyl alcohol (30:70) and its flow rate is 1.2ml/min, the detecting wave is 240nm, and the sample size is 10l.Results1. Methomyl ingredient, the main content of carbamates insecticide, was detected in the tissue treated with formalin and the separating effect is satisfactory. The Guinea pigs in experimental group were given Methomyl (100mg.kg-1). The viscera samples were treated in different ways and then Methomyl content was detected postmortem. The results are list as follows :17.09±1.56g·ml–1 in blood in heart, 1.89±0.08-2.90±0.18g·g–1 in fresh tissue and 1.67±0.17-2.69±0.18g·g–1 in formalin fixed tissue. The LOD of biosample is 0.045mg.kg-1, RSD% in one day and between days are both less than 5.2%. The absolute rate of recovery for formalin fixed biosample is(97.76±0.86)% , the relative rate of recovery is(96.92±0.33)% and abstracted rate of recovery is(94.74±2.88)%. The biology elimination rate is%?...
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