Preliminary Study On MicroRNA Expression Profiles Derived From Formalin-fixed Paraffin-embedded Tissue Of Henoch-schonlein Purpura Nephritis

Objective To establish micro RNA(mi RNA)differential expression profiles of Henoch-Schonlein purpura nephritis(HSPN),using archived formalin-fixed paraffin-embedded(FFPE)tissue,and to provide the evidence that mi RNAs are involved in the molecular pathogenesis of HSPN.Methods FFPE renal biopsy tissue sections from 117 children with HSPN and normal renal tissue sections from 5 nephrectomized children for nephroma used as control group were examined for human mi RNAs by Taqman Low Density Arrays(TLDA)respectively.The date were analyzed by bioinformatics methods: differential mi RNAs expression analysis,target gene ontology(GO)analysis and pathway analysis.The mi RNAs and target genes predominating in the mi RNAs-genes regulatory net work was further investigated.Results Using 3-fold expression difference as a cutoff level,we identified fourteen mi RNAs significantly differentially expressed between HSPN and normal tissues.Two mi RNAs were up-regulated including hsa-mir-222* and hsa-mir-31,while twelve mi RNAs were down-regulated including hsa-let-7a,hsa-let-7e,hsa-mir-10 a,hsa-mir-101,hsa-mir-125 b,hsa-mir-130 a,hsa-mir-138,hsa-mir-145,hsa-mir-184,hsa-mir-409-3p,hsa-mir-21*,and hsa-mir-411.A total of 1662 target genes were predicted by bioinformatic methods.According to the GO and Pathway analysis,categories of high enrichment GOs and specific pathways targeted by deregulation mi RNAs were found involving HSPN.Furthermore,mi RNAs-genes net work was performer successfully.Conclusions There were differentially expressed mi RNAs in HSPN and normal tissue.These mi RNAs mitght be correlated to the pathogenesis of HSPN.According to the bioinformatic techniques,these mi RNAs might regulate their target genes through several pathways and then affect the occurrence and development of HSPN.