Experimental Study Of BYHWD Pharmacology Effect On The Stroke Sequelae Models Of Rats Manifested As Syndrome Of Qi Deficiency And Blood Stasis

Objective: On the base of theoretic studies of BYHWD, combining prescriptions and syndromes and through replicating the stroke sequelae models of rats manifested as syndrome of qi deficiency and blood stasis,we studied the pharmacology effects and action mechanisms of BYHWD on treatment the stroke sequelae models of rats in order to offer the bases for clinic and the clues for the further studies.Methods: BYHWD was processed with traditional methods; Astragaloside IV was the main composition of supplying gas and ferulic acid was the main composition of promoting blood circulation of BYHWD.We selected astragaloside IV and ferulic acid as the quality control standards together.Detected astragaloside IV with the eveaporative light scattering detector and ferulic acid with UV one of high performance liquid chromatography;Replicated the stroke sequelae models of rats manifested as syndrome of qi deficiency and blood stasis. We seleceted the behavior changes,the deficiency grades of nerval founctions,the exterior representations of qi deficiency and blood stasis.the infarct volume of brains and the pathology changes observed from light and electricity lens as the basal indexes of determinant the models coming into existence. Grouped 72 Wistar rats into the nomal group,the control group.the low dosagegroup(12.5g/kg),the middle dosage group(25g/kg),the high dosage group(50g/kg) and the infarct ratio estimated group ransdomly.The rats were treated with BYHWD and 15 days later they were killed.Adopted atom spectrophotometer to assay the Na+,K+,Ca2+,Mg2+ content in the brains of the models,adopted high performance liquid chromatography methods to assay the ATP,ADP and monoamin neurotransmitters(NE,DA,5-HT)content in the brains of the models, and adopted correlative methods to determine such indexes as the whole blood viscosity,blood plasma viscosity,RBC deforrnability,RBC and platelet aggregation etc. which content in the blood of the models. Results:(1) The good linear correlation of astragaloside IV was observed from 1.08 u g to 12.96 u g(r =0.9995)and that of ferulic acid was observed from 0.4 n g to 4.0 u g(r =0.9995).(2) There were significant differences(p<0.01) in each BYHWD treated group about the deficiency grades of nerval founctions at 35d compared with those of 20d.The body hemiplegy syndromes of the middle and low dosage groups were improved greatly. Their weighth were increased,their hair relative lenitive and they acted smartly.The body hemiplegy symptoms of the high dosage group were recovered too but they were relative depressed and their stool was watery.The body hemiplegy symptoms of the control group were not reformed obviously and the wounds were indolence.(3) There were significant differences(p<0.001~0.05)in every other index except the high dosage group having no marked effects on the whole blood viscosity at 5s"1 30 s"1. Compared with the normal group there were significant differences(p<0.001 ~ 0.05)in ADP induced blood platelet congregation in each group and compared with the middle dosage group,there was significant difference(p<0.05)in the high dosage group of it.(4) Compared with the control group,there were significant differences(p<0.05~0.01)in Na+,Ca2+and Mg2+contented in the brains of themiddle dosage and compared with the normal group there was significant difference(p<0.05)in Na+ contented in the brains of the control group.(5) Compared with the nomal group, there were significant differences(p<0.001)in ATP and ADP contented in the brains of the control group and compared with the control group there were significant differences(p<0.001~0.01)in ATP and ADP contented in the brains of each treated group.(6) Compared with the nomal group,there were significant differences(p<0.001)in NE.DA and 5-HT contented in the brains of the control group and compared with the control group there were significant differences(p<0.05~0.01)in NE,DA and 5-HT contented in the brain of each treated g...