Effects Of FS On Liver Regeneration Of Partial Orthotopic Transplanted Rats

Background Shortage of the liver is becoming one of the main reasons hindering the development of liver transplantation with the improvement of liver transplantation technology and the development of operation' s indication. POLT is an effective method to solve the problem. In the field, study on liver regeneration after POLT has been a little and it need to do much more.Objective To develop and modify a rat model of partial liver transplantation with hepatic rearterialization and to evaluate the effects of FS on liver regeneration of partial orthotopic liver transplanted rats. Methods (1) Two-cuff technique described by Kamada and Howden was applied with some modification and hepatic rearterialization between the donor's celiac artery and the recipient s right renal artery was performed in SD rats. The biopsy of graft was adopted at 24 , 48 , 96 and 168 hours after transplantation. (2) On the basis of the model, recipients were divided into two groups: experimental group and control group. Each group contains 24 rats. In experimental rats, 7ug/kg rat body weight of FS was infused into the portal vein via the ileocolic vein immediatelyafter liver grafts were transplanted to the rats, and the same doses of FS were infused via the right jugular vein one a day until the day the rats were killed. In control rats, same vehicle of saline was infused via the ileocolic vein and the right jugular vein. (3) Six rats in each group were killed randomly at 24, 48, 96 and 168 hours. Serum concentrations of albumin, glucose and alanine aminotransferase were determined. Changes in body weight, remnant liver weight, liver regeneration rate and regenerative response of graft were measured.Results: (1) 85.4% of the recipient had survived for one week. At 48 and 96 hours after transplantation, the histopathologic studies of the graft showed the nucleus mitoses occurred. The diploid hepatocytes were found at 168 hours. (2) Serum concentrations of albumin and glucose remained reduced for up to 96 hours in control rats but recovered in follistation-treated rats. (3) In follistation-treated rats, the serum concentrations of ALT was lower than control at 96 hours, while there was no significant difference between follistation-treated rats and control at 24, 48 and 168 hours. (4) In follistation-treated rats, both remnant liver weight and liver regeneration rate were significantly greater at 96 hours. (5) The proliferate index and Ki-67 labelling index of the graft in follistation-treated rats higher than that in control at 48 and 96 hours after transplantation. Conclusions: (1) The modification of the technique provides a morepractical and stable experimental model and liver biopsy in rats is simple and convenient for the study of liver transplantation. (2) It is possible hepatic rearterialization promote the regeneration of the graft. (3) Administering FS after POLT improve the restoration of graft function and promote protein synthesis in rats. (4) Treatment of FS significantly augments the regeneration of graft after POLT in rats.