| Objective:As population in many countries have become increasingly longer and older , the incidence rate of osteoporosis and concomitant diseases related old have increased. Preasently, osteoporosis has confronted 0. 2 billion people and its incidence rate has got the seventh in common disease. The old and particular the old women has apt to got osteoporosis than other people. After the wonen entering menopause, the function of ovary decrease,so the level of oestrogen decrease quickly and hasten the bone reduction. Rescently, study about oestrogen-like and osteoporosis prevention of soybean isoflavone become popular. In this study, we observed biochemical markers of bone turnover, bone mineral density(BMD), bone morphometry and other guidelines to estimate comprehensively the effect of Soybean Isoflavone (SI ) on bone in ovariectomized rats.Methods: Fourty female Wistar rats with 3. 5months old were divided into following groups by body weight randomly:sham group(S),ovx group(0), ovx + low SI group(L),ovx + middle SI group(M),ovx + high SI group (H). All rats feed and water freely after ovariectomized. Recording food consumption and body weight alteration by every week. At the end of 13w, all of rats were killed. Collecting blood for applying tests for serum biochemical markers and serum osteocalcin(BGP); extracting double uteri, liver, nates and spleen to cipher organic coefficient. Lumbar spine, bilateral tibia and femur were dissected out. The bone density (BMD) of total femur and L1-4 lumbar were measured by dual energy X-ray absorptiomtry(DEXA). One of two femur was roasted and cremated, recording dry weight, ash weight and ciphering the ratio of dry weight/ash weight. Undecalcif ied specimen section of the proximal tibia were made to performe bone morphometry abtaining histomorphometric and dynamic data.Result: (l)body weight , food consumption and food utilization:At first of experiment, body weight of each group has no statistical significance, but at the end of 3w , 6w and 9w, there are significant different in each group . By the end of 3w 0 group was highter than other groups and M group was higher than H group;by the end of 6w , M, H and S groups were all lower than 0 and L groups; But at the end of 9w, H and S groups were lower than 0 and L groups. At the end of experiment, 0, L and M groups were all higher than S group, and there is no significance between H and S group. Food consumption of each group have no significance. Food utlization in 0, L, and M groups were higher than S group but there is no significant different between H and S group. (2) Organic coefficient:Besides uterine coefficent has ignificance, other organic coefficent have significant different in each group. Uterine coefficient of S group was higher than other groups and H group was highter than 0 group. (3)Biochemical markers of bone turnover, serum BGP and urinary calcium: At the first of study, all of guidelines were no statistical significance but at the end of experiment, serum calcium of 0, L and M groups were all higher than H and S group. Serum BGP of 0 group was lower than S group and M group. Urinary calcium of 0 and L groups were higher than other groups. (4) Dry and ash weight of femur and ratio of ash/dry:All these variblesof each group have no statististic significance. (5)BMD of femur and lumbar :M, H and S groups were higher than 0 and L group in these two varibles. (6) Bone morphometry : Histomorphometric data suggested following result:TBV/TTV of 0, L and H groups were lower than H and S group;TBV/SBV of 0, L and M groups were all lower than H and S group but M group was higher than 0 and L group. MTPD of M, H and S group were higher than 0 group but H and S group were higher than L group. MTPT of 0 group was higher than other groups but L group was higher than H and S groups. There is no significance in TBS/TBV and MTPT of each group. From dynamic data we can conclude this conclusion: Compared to S group,0 group has a increase in TOS, Sfract (s), Sfract (s/2+d), Svf and decrease in OMP. Compared to 0 g...
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