Effects Of Ketamine On Survival Rate And Serum Concentrations Of Tumor Necrosis Factor-α, Interleukin-6, Nitric Oxide In Septic Rats

Objective: To investigate the effect of ketamine on sepsis induced by cecal ligatione-perforation ( CLP ) and the possible underlying mechanism. Methods: 120 male SD rats were randomly allocated into three groups:(1)normal control group;(2)control group;(3)ketamine group. Each group was again divided into four subgroups (I , II, III> IV). A cecum ligation and puncture procedure was used to make sepsis model. After half an hour of operation, ketamine group was administered with ketamine 50mg/kg every 12 h intramuscularly, whereas normal control group and control group were given equivalent volume normal saline until rats died or 5-day observation ended. The survival period was observed in each subgroups I during 5 days. Arterial blood samples were taken from rats in subgroups IK III IV for measurements of tumor necrosis factor-# ( TNF-a ), interleukin-6 ( IL-6 ) and nitric oxide ( NO )at 0 hour before operation, 4 hours after operation and the dying point of each rat of control subgroup IV. Results: CD TNF-# in control group significantly elevated after the CLP procedure comparing with those of normal '-control group (P < 0.01). In control group, TNF-α concentration at 4h after the CLP procedure was higher than that at the dying point; ㊣L-6 in control group significantly elevated after the CLP procedure comparing with those of normal control group (P<0.01). Moreover IL-6 levels increased in parallel with the timeelapsed;(3) NO in control group significantly elevated at the dying point comparing with that of normal control group (P < 0.01') ; (4) Decreases in the levels of TNF-a and IL-6 were found anytime after ketamine intervention (P < 0. 01) ;(5) NO in ketamine group significantly decreased at the dying point comparing with that of control group (P< 0. 01) ;㏕he 5-day survival rate , in normal control group was 100%, in control group was zero, in ketamine group was 60%. A significantly higher survival rate was found in ketamine group than in control group ( P < 0. 01). Conclusion: Ketamine could restrain the TNF- a , IL-6 and iNO serum concentrations by the CLP procedure and raise the survival rate of rats with sepsis .