The Effect Of Basic Fibroblast Growth Factor And Chitosan On Human Periodontal Ligament Fibroblasts

objectrve:The aim of our study is to cultivate primary human periodontal ligament fibroblasts, to increase the successful rate of primary culture of human periodontal ligament fibroblasts, and to assess the effect of bFGF and chitosan(a water soluble derivation) on human periodontal ligament cells(HPDLFs). Methods: Human HPDLFs were isolated from healthy periodontal ligaments of first premolar teeth of individuals undergoing tooth extraction for orthodontic treatmentThe tissue was mechanically scraped from the surface of tooth root and cells were prepared by using tissue explant culture technique. The pollution of culture was controlled by dividing tooth into crown and root parts,then examined the effect of chitosan on proliferation ,alkaline phosphatase activity ,osteocaclin synthesis of HPDLFs,and examined the effect of human reconbinant bFGF and chitosan on proliferation ,alkaline phosphatase activity osteocaclin synthesis of HPDLFs. Results:Controlling tissue polluted is effective by separating root from crown. The success rate of primary culture of human periodontal ligament fibroblasts was increased. bFGF alone group enhanced the proliferation of HPDLFs,but inhibited the induction of alkaline phosphatase activity..Chitosan alone groups inhibited the proliferation of HPDLFs,but chitosan enhanced alkaline phosphatase activity. Combination group(10ng/ml bFGF and chitosan ) have higher proliferation of HPDLFs ,the higer alkaline phosphatase activity and osteocacline synthesis. Conclusions: Chitosan can stimulate HPDLFs to express some of osteoblasticphenotype,bFGF incorporated into chitosan may upregulate the proliferation of HPDLFs , stimulate HPDLFs to become osteoblasts. Chitosan and bFGF-boaded chitosan may be beneficial to enhance periodontal regeneration.