Experimental Research On The Effect Of Lower-Power Laser Irradiation On Activity Of Alkaline Phosphatase Of Cultured Human Periodontal Ligament Fibroblasts

BAKCGRUNG AND OBJECTIVE: Periodontal ligament fibroblasts were regarded as an important cell that takes a key role in the regeneration of periodontal tissue. Because of their ability of mineralization in vitro and the reaction to many growth factors, periodontal ligament fibroblasts were seen as cell that has potential ability to form bone and are important cells in the study about periodontal regeneration. The aim of our study is to cultivate primary human periodontal ligament fibroblasts, and to increase the success rate of primary culture of human periodontal ligament fibroblasts. METHODS: Human premolars were isolated from 12-14 year old adolescents who require orthodontic treatment. The tissue was mechanically scraped from the surface of tooth root andcells were prepared by using tissue explant culture technique. The pollution of culture was controlled by dividing tooth into crown and root parts. RESULTS: Controlling tissue polluted is effective by separating root from crown. The success rate of primary culture of human periodontal ligament fibroblast was increased. CONCLUSION: The cultured cells are human periodontal ligament cells, which would facilitate further studies.