Expression And Clinical Significance Of P63 And P73 Gene In Laryngeal Squamous Cell Carcinoma

[ObjectiveJ To investigate the expression and significance of p63 and p73 gene in laryngeal squamous cell carcinoma[LSCC].[Methods] The expression of p63a (p51B) mRNAandp63y (p51A) mRNA, p73 mRNA were detected in 43 human laryngeal squamous cell carcinoma tissues, 17 adjacent normal tissues by Reverse Transcriptase Polymerase Chain Reaction(RT-PCR). [Results] The expression of p63y (p51A) mRNA and p63a (p51B) mRNA were found in all detected tissues.The relative intensity of the expression of p63y (p51A) mRNA is 0.87?.30 in laryngeal squamous cell carcinoma tissues,compare to 0.54?.27 of that in adjacent normal tissues.The relative intensity of the expression of p63a (p51B) mRNA is 0.64?.27 in laryngeal squamous cell carcinoma tissues,compare to 0.51?.21 of that in adjacent normal tissues. The expression of p63y (p51 A) mRNA and p63a (p5 IB) mRNA in laryngeal squamous cell carcinoma tissues were much higher than that in adjacent normal tissues (t=3.94, t=1.78,p<0.05). The expression of p63y (p51A) mRNA in laryngeal squamous cell carcinoma tissues were much stronger than that of p63a (p51B) mRNA (t=3.74, p<0.05).No relationship was found between the expression of p63mRNA and the clinical,histologic characteristics. Overexpression of p73 mRNA were found in 26 laryngeal squamous cell carcinoma tissues of 43 laryngeal squamous cell carcinoma tissues and in 4 adjacent normal tissues of 17 adjacent normal tissues.The positive expression rate of p73 mRNA in laryngeal squamous cell carcinoma tissues was significantly higher than that in adjacent normal tissues (x2=6.65,p<0.05).The positive expression rate of p73 mRNA in laryngeal squamous cell carcinoma tissues of stage III~IV group (17/20=85%) was significantly higher (x2=9.42 ,p<0.05)than that of stage I ~ II group (9/23=39%) . [Conclusions] (1)There is an overexpression of p63y(p51A), p63a(p51B)mRNA in LSCC. The expression of p63y (p51A) mRNA was stronger than that of p63a (p51B) mRNA in LSCC. No correlation of p63y (p51A X p63a (p51B ) mRNA expression has been found with clinical, histologic characteristics of LSCC.p63 gene may be one of vital markers of tumors of squamous epithelia origin.(2)There is an overexpression of p73mRNA in LSCC.The expression of p73 mRNA was inversely correlated with the staging of TNM of LSCC, the positive expression rate of p73 mRNA in LSCC of stage III~IV group was significantlyhigher than that of stage I ~ II group. p73 gene positive expression may play a part in LSCC oncogenesis and development and function as an oncogene in the development of LSCC.