Experimental Study On Transformation Of GFAP, SOD And NO In Rat Following Traumatic Brain Injury

In this study, immunohistochemical method was performed to observe the change of glial fibriliary acidic protein(GFAP) staining in the brain of rat after experimental brain injury, at the same time, we also observed the transformation of blood SOD and NO levels of the experimental models. The right parietal cortex contusion was made with stab wound and the rat was killed at various survival time( 30min, Ih, 3h, 6h, 12h,ld, 3d, 7d, 14d, 21d). Paraffin sections(4 μ m) were cut coronally of the whole brain and stained with antibody of GFAP, the hemotoxylin eosin(HE) stain was done in the mean time. The staining results was measured quantitatively with computer imaging analysis system. The SOD and NO of blood from jugular vein after centrifuging were also measured.The results demonstrated that:1. At 30min after injury, GFAP positive astrocytes around the wound were already deteched, but tubers were short and staining was light; at 3d after injury, the positive astrocytes were increased, the bodies were bigger and the staining was heavier than before; at 7d after injury, the amount, the tuber, the staining were decreased, no obvious difference was deteched between injuried and normal rats till 21d after injury.2. The difference of every time group of GFAP positive cells in both sides of brain was analysed, at 3d after injury, the difference was distinct, at the other survival time after injury, there was no obvious difference with the normal control group.3. The GFAP positive cells were observed in the two sides of the brain and no obvious difference between injuried and non-injuried sides was deteched.4. At 30min after injury, the activity of SOD decreased, came to the lowest point at the time of 7d, then increased, at 21 days after injury, there was no obvious difference with the normal control.5. The density of NO came to lowest at the time of 3h, then increased, came to peak at 7 days; at 21 days after injury, there was no obvious difference with the normal control.The results of this study indicate there is a rule that the expression of GFAP positivestaining changes and the transformation of SOD and NO of serum with prolonging survival time after brain injury, which will be of great value in estimation of brain trauma time. Especially, the three is relative to each other, which may will promote the accuracy of estimation if we apply the three in the same time.