The Levels Of Oxidative Stress, The Peripheral Arteriographic Characteristics And The Effects Of Interventional Transfusion Of Batroxobin On The Local Oxidative Stress In The ASO Patients

Objective: 1,In order to analyze the levels of oxidative stress and the peripheral arteriographic characteristics and clinical stages in the ASO patients with and without type 2 diabetes mellitus. 2,To investigate the effects of interventional transfusion of Batroxobin on plasma fibrinogen and the oxidative stress in the localization of atherosclerotic lesions in the ASO patients.Methods: 1,To analyze the levels of oxidative stress and the peripheral arteriographic characteristics in the thirty ASO patients with type 2 diabetes mellitus and compared with thirty nondiabetic ASO patients. All cases were confirmed by arteriography. Venous blood samples were collected in the morning after an overnight fast .The concentrations of malondialdehyde in erythrocytes (E-MDA) and the activities of superoxide dismutase in erythrocytes (E-SOD) were measured by colorimetric method. the clinical data and the arteriographic characteristics were analyzed. 2,Forty-two ASO patients confirmed by arteriography were divided into two groups at random:the Batroxobin group(twenty-two patients) received Batroxobin and heparinized saline via microcatheter, the control group (twenty patients) received heparinized saline via microcatheter at the same dosage and speed. Blood samples were collected before and after the treatment via microcatheter. Colorimetric method was used to measure the concentrations of E-MDA and the activities of E-SOD. Serum plasma fibrinogen was measured before and after the treatment .Result: 1,In comparison with the nondiabetes group, the results showed the significantly higher concentrations of E-MDA [(3.1±0.9 )nmol/ml vs (2.4±0.6)nmol/ml , P<0.01] and the significantly lower activities of E-SOD [(14.7±1.6)×103NU/gHb vs(17.5±1.6)×103NU/gHb, P<0.01 ] in the type 2 diabetes mellitus group. Significant differences were found in the numbers of main lower limb artery involved the severity of culprit lesion and the clinical stage between two groups . 2,After the treatment ,the concentrations of plasma fibrinogen and E-MDA were decreased significantly [(3.5±0.8g/L vs 2.3±0.8 g/L,P<0.01 and(3.1±0.7)nmol/ml vs(2.1±0.5)nmol/ml,P<0.01],and the activities of E-SOD were improved [(18.5±2.4)×103NU/gHb vs(21.8±1.6)×103NU/gHb,P<0.01 ] in the Batroxobin group. There wre no significant differences in the concentrations of plasma fibrinogen and E-MDA and the activities of E-SOD in the control group.Conclusion: 1,Compared with nondiabetes mellitus ,there were higher levels of oxidative stress and more severe and extensive of peripheral arterial disease in the ASO patients with type 2 diabetes mellitus. 2,Interventional transfusion of Batroxobin decreased the concentrations of plasma fibrinogen and the level of oxidative stress in the localization of atherosclerotic lesions in the ASO patients.