The Study Of Immue Function Of Dendritic Cells In Tissues Of Human Normal Brain And Glioma

Background and objective: High levels of CD80 and CD86 play an important role in interaction between immature T cell and DC as second costimulatory signal. CD83 was proved to be a special mark of matured and functional DC. The impaired immunology funtion of DC and the inadequate costimulatory B7 molecule expression may contribute to the escape of tumors from immune surveillance. In this study, we try to use immunohistochemistry staining method to observe the expression of CD80, CD86, CD83 and S-100 in normal brain tissue and glioma tissue. Moreover, we also try to find if matured and functional dendritic cells as well as costimulatory B7 pathway work in glioma.Methods: All the specimens were obtained during operation and diagnose by clinical criteria and confirmed by appropriate histological findings (hematoxylin and eosin staining). All the specimens were from The 2nd Affiliated Hospital of Shantou University Medical College, Shantou, China 515041 and The Center Hospital of Shantou City, Shantou, China 515031. At the same time, all the samples were fixed in 10% buffered formalin, embedded in paraffin, and cut into 4 u m section. Immunohistochemical staining was performed. We detect CD80,CD86 and S-100 by using SP means and detect CD83 by using SAP means.Results: The positive rates of S-100 were separately 100% in the tree groups, the AGV (Average Positive Gray Scale Value) of positive stain were 72.20+12.30, 35.60+9.50 and 28.60+10.20 separately in human glioma tissues, paraglioma tissuesand normal brain tissues measured by analysis system of type HPISA-1000. The difference between glioma tissues and normal brain tissues was statistically significant (P<0.05), but there was no difference between normal brain tissues and paraglioma tissues (P>0.05). No expressions of CD80 , CD86 and CD83 were detected in paraglioma tissues and normal brain tissues. No expression of CD80 and CD83 were detected in glioma, just only 1 section of glioma was weakly positive of CD86.Conclusion: Our findings suggest The up-regulation expression of S-100 in human glioma indicates that S-100 protein is closely relate to gliocyte proliferation and the pathogenesis of glioma. However, in the study of tissues of human normal brain and brain diseases, S-100 can't be treated as a mark of DCs. Matured and functional and positive CD83 DCs were never dectected in our study in tissues of normal brain and glioma. As to CD80 and CD86, whether expressing in tissues of normal brain and glioma or not, we suggested they must be explored further and further.