Influence Of Qilingyiganjian On Expressions Of P21 And CDK4 In Ascites Hepatoma Cell Line H22 And Clinical Study

Objective: To study the resistant function of QilingYiganjian(QLYGJ) to ascites hepatic carcinoma, influence to the expression of P21WAF1/CIP1 and CDK4, and the clinical study of QLYGJ to malignant tumor to discuss the possible mechanism of tumor resistance.Methods:P21 and CDK4 are examined by using immunohisto- chemistry staining method in the tumor tissues of 24 mice; immune indicators of 28 clinical malignant tumor patients before and after using QLYGJ.Results: 1. The experiment of QLYGJ and LZJJ resistance to growth of primary hepatic carcinoma of mice of QLYGJ and LZJJ shows:Tumor weight of control group (n=8) is 0.7331±0.0603g, while the tumor weight of QLYGJ group (n=8) is 0.4668±0.0523g,the resistant rate is 36.33%, it is obviously lower than control group (P<0.01). Tumor weight of LZJJ group (n=8) is 0.5558±0.0803g, the resistant rate is 24.18%, it is obviously lower than control group(P<0.05). 2.Expression of P21 in Hca-F25/CL-16A3 cells through immunohistochemistry staining.Three groups have positive expression. Rank sum test show that the expression of P21 in QLYGJ group is increased and apparently different from that of control group (P<0.01), while LZJJ has no effect on the expression of P21 and is not different from that of control group (P>0.05).3.Expression of CDK4 in Hca-F25/CL-16A3 cells through immunohistochemistry staining: Three groups have positive expression. Rank sum test show that the expression of CDk4 in QLYGJ group is decreased and appareatly different from that of control group (P<0.01), while LZJJ has no effect on the expression of P21 and is not different from that of control group (P>0.05).4.After QLYGJ to 28 clinical malignant tumor patients,the effective rate is 3.6% and the clinical response rate is 85.7%, immune indicators all increase and performance status all improve. Conclusions:1.QLYGJ and LZJJ have apparently inhibiting effects on ascites hepatoma cell line Hca-F25/CL-16A3 cells, and QLYGJ group is higher than LZJJ group.2.QLYGJ can increase the expression of P21 and decrease the expression of CDK4 on ascites hepatoma cell line Hca- F25/CL-16A3 cells, while LZJJ has no effect on the expression of them.3.The clinical study shows that QLYGJ has some function of tumor resistance.