Expression Of PTEN And CDK4 In Human Bladder Transitional Cell Carcinoma And Its Clinical Significance

Objective: To investigate the expression of PTEN, CDK4 protein in bladder transitional cell carcinoma ( BTCC ) and the relation between their expression and carcinogensis, progression and clinical pathological parameters.Methods: A specifically and sensitively immunohistochemical S-P assay was used to detect the expression of PTEN and CDK4 in paraffin-embedded sections of 40 cases of BTCC and 10 normal bladder mucosa.Result: 1. The positive rates of PTEN protein expression were 67.50% in BTCC tissues and 100% in normal bladder mucosa tissues. There was significant difference between them(P<0.05).2. The positive rates of PTEN protein expression in G1, G2 and G3 were 89.47%, 53.85ï¼…, 37.50ï¼…,respectively; and the difference was statistically significant between G1 and G2 or G3(P<0.05).The positive rate of PTEN protein expression in Tis-T1 was significantly higher than that in T2-T4(P<0.05). The PTEN protein expression deletion rates in BTCC were significantly associated with histological grades and clinical stages. 3. The positive rates of CDK4 protein expression were 52.50% in BTCC tissues and 0.00% in normal bladder mucosa tissues, and the difference was statistically significant between them(P<0.05). 4. The positive rates of CDK4 protein expression in G1, G2 and G3 were 36.84%, 61.54ï¼…,75.00ï¼…,respectively; and there wasn't statistically significant difference among the three groups (P>0.05).The positive rate of CDK4 protein expression in Tis -T1 was significantly lower than that in T2-T4(P<0.05). The CDK4 protein overexpression rates in BTCC were significantly associated with clinical stages.Conclusion: PTEN protein expression deletion and CDK4 protein overexpression are closely related to the occurrence and development of BTCC. To detect PTEN and CDK4 protein together is an important method for the evaluation of the malignancy and prognosis of BTCC.