Researching Of The Relationship Between The P130 Gene Expression And Nasopharyngeal Carcinoma

The Cancer is one of the most serious diseases endangering humanbeing's life and health. And the "Canton Tumor"-nasopharyngealcarcinoma ( NPC ) has district aggregation and race munity, its pathogenic factor is related to the infection of EBV, heredities, environment and diet factors, and it has the characters of secretive morbidity, early metastasis and easy relapse.With the developing of modern molecular biology, the cancer ( including NPC ) is deemed to be closely related to many oncogenes and antioncogenes. This research is focusing on the relation between antioncogene of p130 and nasopharyngeal carcinoma. The p130 gene is one of the members of Retinoblastoma gene family. It is locating human chromosome 16ql2.2, contains 22 exons, and expresses widely in all kinds of normal human tissues. Its production expressed has 1139 aminoacids, with the A/B pocket domain that may interact with virus oncoprotein, transcription factor of E2F, cyclins or its dependent kinases et al.. The p130 gene participates in and maintains the transcription sleep of the phase of GO of cytodiaeresis cycle, and inhibits the growth of the cells. It correlates with the onset and progress of a great variety of the tumors. And it was found that there were mutations of the p130 gene in NPC by foreign researchers.We analysed the p130 of the tissue sectio of paraffin embedding that was pathologically diagnosed as nasopharyngeal carcinoma by immunohistochemistry, with the chronic nasopharyngitis tissues as the control group, and found that the positive ratio of p130 in NPC group is lower than the chronic nasopharyngitis group (P<0.05). We also collected nasopharyngeal tissues of biopsy ( including NPC and chronic nasopharyngitis tissues ) and isolated RNA and DNA, then detected the quantity of p130 gene mRNA by real time fluorescent quantitation RT-PCR, finding that the quantity of p130 gene mRNA in NPC group is lower than the chronic nasopharyngitis group (P<0.01); and at last we detected the deletions and mutations of the 11th, 17thand 19th exons of p130 gene, finding that there are 3 samples with the deletions of the 11th and 17th exons and 6 sampleswith the mutations of the 19th exon.The research of the low expression of p130 gene in NPC tissues and the deletions or mutations of its exons, and further research of the relations between the pi30 gene and other malignant tumors will be probative for the breakthrough of carcinogenic mechanism researching, and may be the base of gene therapy-the new therapy of cancer in the future.