Separation And Analysis Of Acidic Oligosaccharides By High Performance Capillary Electrophoresis

Alginate oligosaccharides is a kind of marine acidic oligosaccharides made of -1,4-D-mannuronic acid and -1,4-L- guluronic acid with good anti-virus, anti-tumor and anti-inflammation activities. In this paper, alginate oligosaccharides are separated and analyzed by high performance capillary electrophoresis.Alginate oligosaccharides degraded by the alginate lyase are separated by HPCE with UV 235nm detection. CE separation conditions have been investigated. The oligosaccharides are analyzed in conditions of pH 2.5, 100mmol/L phosphate buffer, 20kV, temperature 30 C. In this condition alginate oligosaccharides are successfully separated.Alginate oligosaccharides degraded by acid are derivatized with 8-aminopyrene-l,3,6-trisultonate (APTS) by reductive animation and their ARTS derivatives are separated and detected by capillary zone electrophoresis with laser-induced fluorescence. Opitimization studies have also been performed with respect to labeling and HPCE separation conditions of oligosaccharides. The detection sensitivity can reach 10~17mol, standard curve and linearity range are determined. The alginate oligosaccharides- APTS derivatives are separated in an acidic phosphate buffer (pH 2.5) and in the alkaline borate buffer (pH 10.02), respectively. Acid hydrolysis products of alginate labeled with APTS are successfully mapped and the M/G ratio of alginate is also determined by this method.There are some comparabilities in structure and the properties of electrification between marine acidic oligosaccharides and endogenous substances, so the study of acidic oligosaccharides by HPCE has important significance.