Study Of Co-stimulatory Molecules Mediating The Immunopathology And Molecular Mechanism Of The Mice Infected With Schistosoma Japonicum

Recent studies showed that regulating Th1/Th2 immunodeviation is a effective method to control granulomatous formation. The activation of T cells required two signals. The second signal is co-stimulatory which can regulates Th cell polarization and cytokine secretion. B7-1/2:CD28 and CD40:CD40L are two kinds of important co-stimulatory signals of T cell activation. In this paper, through observing the regulating effects on Thl/Th2 immunodeviation to interfere with the two kinds of co-stimulatory signals, we probed the effect on eggs granulomatous formation. The results showed that the Anti-B7-lmAb and Anti-B7-2mAb could not significantly inhibit the IFN- Y expression (P>0.01). But they could significantly inhibit the expression of IL-4 (P<0. 01), and the effect of Anti-B7-2mAb is stronger than that of Anti-B7-lmAb. Anti-CD40mAb and Anti-CD40LmAb could also significantly inhibit the levels of IL-4 but not that of IFN-Y . The measure of NO levels further demonstrated above the mechanism. The same time we analyzed the correlation of the expression of B7-1/2 and CD40/CD40L on liver egg granulomatous cells and the granuloma during different infection phases. We further probed the effects of co-stimulatory molecules on the specific Thl/Th2 immunodeviation of Schistosoma japanicum and its immunopathologic mechanism. The results show that the four co-stimulatory molecules anticipate the egg granuloma formation. Especially the effect of B7-2 and CD40 molecules are more significant. Its mechanism is probably the two kinds of molecules mediating Th2 predominant reaction. These results have important theoretic and practical value for finding the new pathway to control eggs granuloma and preventing advanced schistosomiasis.