The Roles Of Activated Nuclear Factor-κB In Severe Acute Pancreatitis And Its Intestinal Mucosal Barrier Injury

Objective: To investigate the roles of activated nuclear factor-kappa B (NF- B) in severe acute pancreatitis (SAP) and the intestinal mucosal barrier injury in SAP.Methods: 72 Wistar rats were randomly divided into sham-operation (SO) group, SAP group, and SAP previously treated with pyrrolidine dithiocarbamate (PDTC) group. SAP was induced in rats by injection of 5% sodium taurocholate into biliopancreatic duct. Plasma TNF- a , IL-6, D-lactate and endotoxin were determined at 1,3,6 and 12 hour after operations. Activated NF- K B in the intestinal mucosa was detected with immunohistochemistry at 1,3,6,12 hour after operations.Results: The plasma cytokine TNF- a and IL-6, D-lactate and endotoxin in SAP group increased obviously compared with SO group (p<0.05). The TNF- a reached its peak at Ih while IL-6 did at 3h postoperatively And the plasma concentrations of D-lactate and endotoxin increased stabilizedly after operations in SAP group. The treatment with an inhibitor of NF- K B activation, PDTC, markedly decreased the plasma TNF- a , IL-6, D-lactate and endotoxin level compared with SAP group (p<0.05), and also ameliorated the histologic injury of pancreas and intestinal mucosa of SAP. Activated NF- K B was almost absent from intestinal mucosa in the SO group but remarkably increased in SAP group ,especially at 3h postoperatively. The degree of NF- K B activation was decreased in intestinal mucosa of PDTC-treated group as compared with SAP group.Conclusions: Activation of NF- K B is one of the major factors for SAP and the intestinal mucosal barrier injury in SAP.