Study On The Quality Standard Of Fennel

Fennel is not only a general-adopted flavouring essence, but also a Chinesemedicine of dispelling cold, relieving pain and regulating the stomach function. We mainly aimed at fennel in china area and founded a higher standard of the quality of fennel through a great deal of studies on source, character,identification and the analysis of compositions.The research can be divided into three sections:One section General research work of FennelFennel,as a tranditional Chinese herb used in common,was first recorded in TangPharmacopoeia.Huaixiang was its old name,it is verified the same as the legal goods(Foeniculum vulgare Mill.).It is planted almost all area in china, especially in Inner Mongolia, Ningxia, Shanxi, Xinjiang. There are some imported goods from southeast Asia.Dill,Geluzi,Tiangeluzi, Ziran are the main fake variety.People distinguish them by observing their outside characters or through microscopical identification and thin-layer chromatography .The components of Fennel are fatty oil, volatile oil, sterol, glucoside, amino acid etc. The major component of Fennel is volatile oil. Processing medicine using heat is beneficial for the extracting of volatile oil. There is a little research work on the component of fennel and less report can be found.Fennel has various pharmacodynamical functions, such as promoting to the movement of stomach and intestines, contradicting ulcer, benefitting gallbladder, paralysing center, relaxing trachea, accelerating the resume of liver and hormonelike function, especially have improving immunity resisting tumour sterilizing bacteriunu falling blood pressure and so on. It is not reported for toxicity and side-effect on glut using, proved to be a safe Chinese traditional medicine. It also has a wide range clinical application.There are 68 compound Chinese traditional medicinecontain this herb.Two section Study on the quality control of volatile oilwe totally collect the following 10 breeds of the domestic crude drug. Fennel crude drug yielded respectively by Inner Mongolia Tuokutuo Town, Taiyuan,Guangdong, Shanghai, Xi'an, Ningxia, Xingjiang, Sichuan, Baoding and Beijing. The above crude drugs are proved to be the ripe fruit of Foeniculum vulgare Mill.The chemical components of the volatile oil in the Fennel from 10 areas were analyzed by GC-MS. The essential oil was extracted from fennel by steam distillation, the components analyzed with the different kinds of capillary columns,the optimum separated and analytical conditions were researched,the amount of the components from the essential oil were determinated by normalization method.The components separated were identified by GC-MS.Chromatographic conditions were : Capillary column HP-5(30m 0.25mm,0.25um);Column temperature :50 (10min) , 2/min to 150(lmin), 5/min to 200(5min); Split injection,split ratio 30:l;Injector temperature 250 .Mass spectrum conditions were:ionization mode:EI,Electron energy70eV,Interface temperature:280 Jonicfountain temperature:200 ,quadropole temperature 100, Mass scan range:35-500AMU, Solvent delay 3.0min. There are 20 components composed of above 94% of the total essential oil were separated and identified. The relatively content of trans-anethole was 65%-78%,it might be a quantify character. Analysed and compared their total ion chromatograms(TIC), the TICs are similar, but there is a little different in the TIC and the relative percentage contents. Their similar components may be used in identification of Fructus foeniculi. The method is reliable,stabilize and good repeatability,this method can be applied to the analysis of the essential oil components was extracted from Chinese traditional medicine components.An assay method was developed for the determination of trans-anethole in fennel by programmed -temperature capillary GC. The volatile oil was extracted from Fructus foeniculi by steam distillation and was analyzed with capillary columns: Capillary column DB-17(15m 0.25mm,0.25um Temp:40 to 280/300),Column temperature is elevated by the program: 50, 5/min to 60, 2...