Study On Quality Control Of Illicium Verum

The dried and ripe fruit of Illicium verum Hook.f.,which is widely used in clinic,is listed in the chinese Pharmacopoeia as an important Traditional Chinese Medicine(TCM).19 samples were collected from different habitats and different butches and the quality control was developed.The present study determine the major biological components in Illicium verum Hook.f.and simultaneously provides the GC fingerprint chromatogram of the essential oil from Illicium verum Hook.f..Subsequently,the systematic quality evaluation of Illicium return Hook.f.was developed.1.The extraction technology of the effective components of the raw materials was studied by an orthogonal design using content of the major effective component the essential oil and the volume of extract as markers,reviewing screen mesh,the solvent quantity,soaking time and the extraction time.2.Gas chromatography was used to establish fingerprint of the essential oil from Illicium verum Hook.f..DB-1 capillary column(30m×0.25 mm×0.25 gin)was used,and the program was 70℃(5 min)2℃/min200℃(5 min)with the FID detection,with the FID detection.19 batches of the raw materials were divided into four grades by the Hierarchical cluster.Mutual pattern was established from 11batches in the gradeⅠandⅡ,the similarity data were also obtained.The chemical composition of the essential oil from Illicium verum Hook.f.was determined by the means of GC-MS.Trans-anethole was more than 80%.3.Trans-anethole was determined by gas chromatography(GC).DB-1 capillary column(30m×0.25 mm×0.25μm)was used,and the program was 70℃(5 min)2℃/min200℃(5 min)with the FID detection.By internal standardization method,the calibration curve of trans-anethole was linear over the range of 0.34～2.38μg/μL(r=0.9998,n=6).Trans-anethole of the volatile oil was determined 63%(w/w)～89%(w/w)in nine batches of samples.The assays were found to be simple,fast and accurate.So they can be used as method to control the quality of Illicium verum Hook.f.