| ObjectiveTo evaluated the effect of Aminoguanidine and Dexamethasone on inducible NOS expression and neurological function in a model of spinal cord injury.MethodThe spinal cord injury model reported by Nystrom was used to result in moderate cord injury (35. 0g, 10min). Wistar rats with an average weight of 230g were randomly divided into following groups; Group A; not injury; Group B; untreated injury; Group C; injury treated with Aminoguanidine; Group D: injury treated with Dexamethasone. At 6,12,24, and 72 hours, after surgery , animals were killed to study the level of iNOS in spinal cord by immunohisto-chemical; At 24,48,72 hours and 1, week, neurological function of hindlimb was assessed by blinded observer using an established multiple test method (motor; toe spread; reflexes to extension, pain; inclined plane and swim test) with tests graded and results expressed as a combined behavioral score.ResultThere was a significant difference among groups in iNOS expression. Group C and D was lower than group B( P =0.008 ,0.007at 24h after surgery). Behavioral scores over time showed a significant difference among four Groups.Conclusion1). The expression of iNOS can be inhibited by Aminoguanidine or Dexamethasone.2). Aminoguanidine and Dexamethasone significandy enhance neurological recovery. |
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