| Flos Carthami is named the dry flowers of a member of the family Compositae or A steraceae commercially. In Chinese traditional medicine theories, Flos Carthami has the effects of activating blood circulation and dredging collateral, Invigorating the Blood and promotes menstruation used for Congealed Blood patterns, with amenorrhea, abdominal pain, postpartum dizziness, lochioschesis, and abdominal masss, dispeling Congealed Blood and alleviating pain used for Congealed Blood wounds and pain Amenorrhea and dysmenorrhea due to blood stasis and postpartum abdominal pain due to retention of blood stasis. And now it is widely used for various kinds of pain due to blood stasis such as thrombosis and so on.In this paper, the fibrinolytic enzyme is produced by a strain of Bacillus licheniformis, and is a multi-component protease. Because of its strong activity of dissolve fibrin directly, it has a prospect of being a medicine for thrombosis. Microbes have great power to decompose and transform substances and can produce abundant second metabolites. Traditional Chinese medicine processed by the growth and metabolizability of microbes, it can get the results such as changingtherapeutic features, promoting effects of medicine, decreasing the toxic action and ;ide effects and increasing indications. The microbe studied in our study is a strain of Bacillus licheniformis called C2-13. It not only grows fast and has simple nutrition demands, but also can produce strong fibrinolytic enzymes and many other enzymes that can do good to the processing of Traditional Chinese medicine such as protease, glycohydrolase, amylase, lipase and so on.In this paper, our research focused on the co-fermentation of Traditional Chinese medicine Carthamus tinctorius L (CTL) and C2-13, the main content include:I the screening, identifying, optimizing of the fermentation conditions of Bacillus licheniformis.II CTL's effects on the growth and metabolizability of Bacillus licheniformis C2-13.III Bacillus licheniformis C2-13's effects on the activities of CTL after processing.IV Primary study on the mechanism of fermentation. The main results of our study include:I Bacillus licheniformis C2-13 was isolated from the sewage of Chengdu shambles. We used the activity of fibrinolytic enzyme as an index, and also used the technology optimizing disposition of the system factors with all over feedback control to optimize the fermentation conditions. The results were as follows: sugar2.0%, yeast extracts 0.5% , KH2PO40.8%, CaCl20.1%, MgSO20.5%, corn powder 0.5%, initial pH 10.0, inoculum size 1.5%, medium 25mL (in 250mL flask) and culture time36h at 39, 180-200r/min.II We chose CTL in different concentrations to co-ferment it with C2-13, used the growth curve method to measure the effect of CTL on the growth of C2-13 and used the activity of fibrinolytic enzyme as an indexto measure the effect of CTL on the metabolizability of C2-13. The results were as follows: within the range of 0~50g/L, the effect of CTL on the growth of C2-13 had a process from promoting to inhibiting. Within the range of 0~30g/L, TCL could promote C2-13's growth greatly and it had an obvious dose-effect relationship. But if the concentration increased, there was inhibitory on the bacteria's growth. At the same time, the fibrinolytic enzyme's activity has also shown a process of rising firstly and then falling.III We studied C2-13's effects on the main medical activities of CTL such as activating blood circulation and dredging collateral, antioxidative activities and so on after processing. The results were as follows: After co-fermentation with C2-13, CTL's antioxidative activities including the ability of anti-oxidation of hydroxyl radical and inhibitory activities of CTL, also the ability of activating blood circulation and dredging collateral had been promoted to different extent.IV We have used HPLC to study the mechanism of the co-fermentation of CTL and C2-13. The results were as follows: there are five samples that have... |
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