Research On Active Peptides From Pilose Antler

In order to elucidate the mechanism of bone-strengthening and anti-tumor ofeffects of pilose antler, extract　of freeze dried pilose antler powder was fractionatedby combination of gel filtration chromatography on Sephacryl Ｓ－２００ＨＲ, ion-exchangechromatography　 on　 ＰＯＲＯＳ　２０ＱＥ, reverse phase chromatography on Ｃ１８　 andchromatography on　ＴＳＫ　Ｇ３０００ＳＷ. As a result three bioactive components, Pb, Ｃ１８－３　and Ｃ１８－Ｃ　were obtained. Ｐｂ, electrophoretically pure, with a molecular weight of ５６．３ｋＤａ significantlyincrease the proliferation rate of　the osteoblast-like ＵＭＲ－１０６ cell and the secretion of　ＩＧＦ－Ｉ. When the concentration of Ｐｂ was　０．１４９μｇ／ｍｌ, the proliferation rate ofＵＭＲ－１０６ cell was　２４１．０３％, and the secretion of　ＩＧＦ－Ｉ　was　６６．８９ｎｇ／ｍｌ. The partialＮ － terminal amino acid sequence of Ｐｂ　 were identified as:ＤＨＫＳＥＩＡＨＲＦＫＤＬＧＥＤＮＦＱＧＬＶＬＩＡＦＳＱＹ, and it was determined to be deer　serum albumin　by the result of sequence comparison. 　Ｃ１８－３ fraction which was proved to be pure by capillary reverse phase wasdemonstrated to inhibit ＵＭＲ－１０６ cell proliferation. The minimum inhibitionconcentration was ０．０１５８μｇ／ｍｌ　with dose-dependence. It is showed that thoseregulative factors in　ＰＡＥ could affect the proliferation of　ＵＭＲ－１０６ cells two-wayly. Ｃ１８－Ｃ fraction which was proved to be pure by capillary reverse phase wasdemonstrated to inhibit the tumor cell ＢＥＬ－７４０２ proliferation． The minimuminhibition concentration was　０．００１８５μｇ／ｍｌ withdose-dependence.