The Experimental Study On Bioeffects Of Interventional Therapy To Tumor-Bearing Nude Mice Of PC-3 Human Pancreatic Carcinoma By Chromic[32p] Phosphate Colloids

Objective To establish tumor xenograft of human pancreatic carcinoma Pc-3 with BALB/c-nu/nu nude mice. To study the biodistribution, pharmacokinetics and toxic reaction of chromic phosphate colloids(Cr32PO4,P-32 colloids)interstitial administration to BALB/c-nu/nu tumor-bearing nude mice of Pc-3 human pancreatic carcinoma.To study the tumor-inhibiting effects of chromic phosphate colloids (Cr32PO4,P-32)intratumoral injection in tumor-bearing nude mice and the relation between dose and anti-tumor effect..Methods Each of 18 nude mice in 51 were injected with 14.8MBq P-32 colloids in 0.05-0.1ml through caudal vein or tumor interstitial injection,and were killed after 2h,24h,48h,72h and 168h,respctively,the radioactive distribution of P-32 colloids in mouse body were observed dynamically.33 nude mice else were intratumoral injected with P-32 colloids of 3.7MBq,7.4MBq,14.8MBq,18.5MBq,29.6MBq and 0MBq, blood were collected in 0-28d after administration, and white blood cells and platelet were counted,body weight and morphology were studied. The effective half life of P-32 colloids was calculated in the way of tumor surface radioactive counts measuring per minute of 12 nude mice everyday. Results Intratumoral administration of P-32 colloids makes high level of radioactivity retained in the tumor, the radioactive counts per minute in tumor were obviously higher than those in other organs or tissues; the organs or tissues radioactive counts per minute after intra- tumor administration were evidently lower than those after caudal vein administration. The effective half life of P-32 colloids was 13 days. Morphology examination demonstrated that most of Pc-3 cells were destroyed after intratumoral administration of P-32 colloids,and benign differentiation tumor cells were detected. The tumor growth inhibition rates are positive correlated to absorption doses.The radiation lesions of liver,spleen, lungs,lymph node and other important organs were reversible. No obvious bone marrow depression was detected.Conclusion It is demonstrated that the intratumoral injection of P-32 colloids is a safe and effective interventional therapy for pancreatic tumor and lymphatic metastasis.