Study Of The Effects Of Vascular Endothelial Growth Factor In Intrahepatic Cholestasis Of Pregnancy And Therapeutic Effects Of Salvia Miltiorrhiza Bge

University: Southeast universityObjective:To explore poor perinatal outcome in ICP and it′s mechanisms responsible for fetal intrauterine asphyxia;To study the mechanism of Salvia Miltiorrhiza Bge(丹参)in improving fetal blood microcirculation in ICP.Methods: Thirty patients with ICP and thirty normal pregnancy women take part in this study.Their periblood is collected and centrifugated.Sixty newborns' weight,stature,Apgar score,placental weight and meconium staining of amniotic fluid are compared.Endothelial cells are isolated from human umbilical veins according to Jaffe′s method.Serum of different concentration of ICP groups and normal pregnancy groups is cocultured with HUVECs for different time point respectively.Salvia Miltiorrhiza Bge of 1,2,4,8mg/ml is used to intervene HUVECs in ICP group.MTT method is applied to measuring the vitality of HUVECs.The expression value of vascular endothelial growth factor(VEGF)of different groups is detected by immunocytochemistry and Enzyme-linked immunosorbent assay (ELISA) respectively.Results: ①Compared with normal pregnancy,neonatal weight and placental weight are markedly lower,the incidence rate of meconium staining of amniotic fluid is distinctly higher in ICP group.②The value of MTT in ICP serum groups is significantly lower than that in the normal pregnancy serum group.With the increase of serum concentration,the value of MTT decreases.③Compared with the normal group,the value of sVEGF in ICP serum group is lower.Furtheremore,the value of sVEGF decreases at most if HUVECS were cocultured with 20%ICP serum for 24 hours.④In the Salvia Miltiorrhiza Bge therapeutic groups,the value of MTT and VEGF increases with the concentration increase,8mg/ml of alvia Miltiorrhiza Bge is the optical concentration and being no significantly different from the normal serum group.⑤By immunocytochemistry,the expression of VEGF in HUVECs decreases in the ICP serum group,8mg/ml of Salvia Miltiorrhiza Bge enhances the expression of VEGF at most. Conclusion: ①ICP had bad effects on the fetus.②Serum of ICP patients impairs HUVECs in vitro, but Salvia Miltiorrhiza Bge could protect HUVECs against the injury of ICP serum.③The serum of ICP patients decreases the expression of VEGF in HUVECs.While Salvia Miltiorrhiza Bge promotes the expression of VEGF.④Salvia Miltiorrhiza Bge improves fetal blood microcirculation in ICP group by increasing the value of VEGF .