| Objective: Periphral nerve regeneration is one of the important subjects of clinic study. Prolonged denervation can result in degeneration and atrophy of target organs and increase the risk of permanent loss of function. The functional recovery of injuried peripheral nerve is not by mitosis of nerve cell, but by repairment and regeneration of axon and myelin sheath. Repairment and regeneration of injuried peripheral nerve involves in very complex pathophysiological process. There is a tight relationship between the injury degree and reasonable medicine. There are many affecting factors, for example the age,ACTH,galactose-cerebroside,the electromagnetic field and the immunosuppressive agent,but the microcirculation and the flow of blood is the foundation of regeneration of injuried nerve .The persent study is to investigate the effect of Lumbar Sympathetic Ganglionectomy(LSGT) therapy on the structure and function recovery of the injuried peripheral nerve using the model of sciatic nerve injury designed by LiYuHao. Method: The experiments were performed on 60 adult SD rats either sex. These animals were divided into two equal groups randomly(n=30). GroupA( the control group): simple crush; Group B (the experimental group): Lumbar Sympathetic Ganglionectomy therapy after crush. The Method of LSGT:Under the condition of asepsis,the tissure of rats is cut open to expose and cut off the sympathectic ganglion,which is under the left renal artery, and then closing up. The skin temperature of the left feet of the rats: Measuring all of the skin temperature of the left feet of the rats before LSGT and after 5 days′LSGT by the ultra-red ray machine. The Sciatic nerve Function Index(SFT), the Sciatic Nerve Conduction Velocity(SNCV) and Wet Weight of Gastrocnemi-us(WWG) were measured at 2,4,6weeks after crush to elucidate the restoration of the injuried nerve. The morphometric tests were also performed using Glees and Marsland's strain, to observe changes of neuromchanism in the speciments including the areas next to the crushed after 2, 4, 6 weeks'Lumbar Sympathetic Ganglionectomy. Superthin slices for electronic microscope after 4 weeks′LSGT. Results: ⑴the skin temperature of the left feet: (before injury)Group A:31.02±0.12oC,Group B:31.04±0.11oC(p>0.05); (after 5days′LSGT) Group A: 31.05±0.10oC, comparing with of the before in Group A, P>0.05;Group B:31.90±0.13oC, comparing with of the before in Group B, P<0.05⑵The Sciatic nerve Function Index(SFT): The SFT in Group B was significantly faster than that in Group A(p<0.01) after Lumbar Sympathetic Ganglionectomy therapy for 2,4,6 weeks.⑶theSciatic Nerve Conduction Velocity(SNCV): The SNCV in Group B was significantly faster than that in Group A(p<0.01) after Lumbar Sympathetic Ganglionectomy therapy for 2, 4 ,6 weeks. ⑷Wet Weight of Gastrocnemius(WWG): The WWG in GroupB was significantly faster than that in Group A(p<0.01) after Lumbar Sympathetic Ganglionectomy therapy for 2, 4 ,6 weeks. ⑸histological findings: After 2, 4 , 6 weeks of Lumbar Sympathetic Ganglionectomy therapy in Group B, at the areas next to the crushed, by Glees and Marsland's strain, lots of regenerating axons and myelinations were observed, structure compacting and fragment lessening. Under electronic microscope, layer structure of myelination was clear, mitochondrion was normal and its number is larger in axoplasm, while Schwann'cell was live, cytoplast being rich and many mitochondria in it. At the same areas in Group A, existing axonal and wallerian degeneration, axon swelling,disordering and parting, segmental demyelination. Under the electrionic microscope, myelination fragmented and mitochondrion swollen. Conclusion: Lumbar Sympathetic Ganglionectomy is beneficial to the structure recovery of peripheral crush injuried nerve. It could accelerate nerve conduction velocity and help restructure nerve normal struture and funtion.
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