| Objective: In recent 20 years, the morbidity and mortalityof colorectal cancer are growing due to the changes of people'sdiet, and therefore, people's health and lives are threatened by it.Progresses have been made in the etiology, the diagnosis and theremedy of cancer, but the probability of metastases andrecurrences still keeps high. Now, early diagnosis and reducingthe recurrences and the metastases become the focus of theresearch.In our research, by studying the genes related to themetastasis in the cancer cells, we try to explore a reliable factor,which can make an early prediction of metastases andrecurrence in colorectal cancer. Gene Kiss-1, found inmelanoma by Lee in 1997, is a new kind of genes which cansuppress the cancer cells. It encodes and produces 54 AAsuppressive factors,which can depress the metastases effectively.According to the past researches, this kind of genes has afunction in the metastasis of the melanoma and breast cancer.And some researches show that gene Kiss-1 has a negativecorrelation on MMP-9. Kiss-1 keeps the NF-κBp65 frommoving to the nucleus by increasing the IkB in Cell HF-1080. Itcan reduce the combination between NF-κBp65and MMP-9promoter, and therefore, reduce the stability level ofMMPmRNA and the synthesis rate rather than degrade DNA. Ithas not been reported in China yet about the expression of GeneKiss-1 in colorectal cancer. Based on the past researches, wetry to study the following : 1) the expression situations of Kiss-1in cancer;2) expressions in different histological grade andlymphatic metastases;3) the correlation among Kiss-1 ,NF-κBp65,MMP-9. The aim is to find out the factor to predictthe colorectal cancer and provide a foundation to predict thetendency of colorectal cancer。Methods: The study included 74 samples of colorectalcancer as experimental group and 10 samples of normalmembrane as control group. According to lymphatic metastasis,the 74 samples could be divided into the lymphatic metastasisgroup and non-lymphatic metastasis group, which included 35samples and 39 samples respectively. According to histologicalgrade,it could be divided into the undifferentiated or poorlydifferentiated group and the well-differentiated group, whichincluded 33 samples and 41 samples respectively. In our study,we detected the expressions of Kiss-1,NF-κBp65,MMP-9 indifferent histological grade and lymphatic metastases by usingthe Flow Cytometry, in situ hybridization and ColorPathological Image Analysis System.Results: 1 In situ hybridization: positive granule weremainly distributed in the cytoplasm of the cancer cells, beingbrown.Kiss-1 expression was obviously higher in colorectalcancer group(82.53±24.85) than that in normal colorectalmembrane group(10.55±3.68),in the lymphatic metastasisgroup(57.25±5.47)it was obviously lower than that in thenon-lymphatic metastases(105.22±6.57)(p<0.05);NF-κBp6expression was much higher in the colorectal cancer group (80.2±23.17) than that in the normal colorectal membranegroup(6.72±4.21),in the lymphatic metastases(102.3±10.37)itwas much higher than that in non-lymphatic metastasisgroup(60.34±8.93),in undifferentiated or poorly differentiatedgroup (89.19 ±22.58) was higher than that in thewell-differentiated group(72.95±21.25)(p<0.05)。2 Flow cytometry: NF-κBp65 expression was obviouslyhigher in colorectal group(FI=4.66±1.68) than that in thenormal membrane group(FI=1.06±0.17),in the lymphaticmetastasis group(FI=5.46±1.68), it was obviously higher thanthat in the non-lymphatic metastasis group(FI=3.94±1.35);inthe undifferentiated or poorly differentiated group(FI=5.69±1.56), it was higher than that in the well-differentiated group(FI=3.86±1.28)(p<0.05);MMP-9 expression is obviouslyhigher in the colorectal cancer group(FI=3.69±1.51)than thatin the normal colorectal membrane group(FI=0.96±0.15),inthe lymphatic metastasis group(FI=4.57±1.45), it wasobviously higher than that in the non-lymphatic metastasesgroup(FI=2.90±1.07)(p<0.05)。3 There was obviously negative correlation between Kiss-1and NF-κBp65(P<0.05)(r=-0.365). There was obviously... |
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