The Relationship Between Expression Of Kiss-1 And CD44v3 And Metastasis In Tongue Squamous Cell Carcinoma

Objective:The study was aimed to explore the expression of Kisspeptin and CD44v3 in primary tumour and lymph node metastatic tumour of tongue squamous cell carcinoma (TSCC) and to explain the relationship between the expression of these two proteins and metastasis as well as its clinical significance in TSCC.Methods:Fifty-nine patients with TSCC were divided into two groups, one group without metastasis containing thirty-eight patients, and the other with lymph node metastasis including twenty-one patients. The group without metastasis was detected primary tumour,and that with lymph node metastasis was detected both primary tumour and metastatic tumour. Kisspeptin and CD44v3 were assessed with immunohistochemistry in primary tumour and metastatic tumour of TSCC. Kiss-1mRNA was detected by in situ hybridization in 59 primarily tumour. SPSS13.0 was used to analysis the relationship between the expression of Kisspeptin, CD44v3, Kiss-1mRNA and metastasis in TSCC.Results:Kisspeptin was observed in 56 cases primary tumour of TSCC, which including 37 cases without metastasis [(+)20,(++)16,(+++)1] and 19 cases with lymph node metastasis [(+)16,(++)3]. The expressive intensity in the group with lymph node metastasis was lower than that without metastasis significantly (p<0.05).There was negative correlation between expression of Kisspeptin and metastasis in TSCC. Expression of Kisspeptin was (-)15 and (+)6 in lymph node metastasis tumour, which was lower than that in primary tumour significantly (p<0.01). Kiss-1mRNA was observed in 55 cases primary tumour of TSCC, which included 38 cases without metastasis [(+)12,(++)22,(+++)4] and 17 cases with metastasis [(+)15,(++)2]. Lower expression levels of Kiss-1mRNA were observed in the group with lymph node metastasis as compared to that without metastasis significantly (p<0.01), which was consistent with that of Kisspeptin. The expression of Kisspeptin was correlated with Kiss-1mRNA without significant difference (r_S=0.535,X~2=0.000). CD44v3 was observed in 56 cases in primary tumour of TSCC, which included 36 cases without metastasis [(+)15, (++)15, (+++)6] and 20 cases with lymph node metastasis [(+)8,(++)10,(+++)2]. There was no statistical difference between the two groups on the expression of CD44v3 (p>0.05). However, the expression of CD44v3 in 21 cases lymph node metastatic tumour [(+)2,(++)12,(+++)7] was higher than its primary tumour significantly(p<0.01). There was no correlation between the expression of CD44v3 and Kisspeptin in TSCC. There was no correlation between the expression of Kisspeptin, CD44v3, Kiss-1mRNA and age, sex, grade of histology, clinical stage, size of tumor, philo-smoke, philo-alcohol separately(p>0.05) .Conclusion:1,Weakness or lost on the expression of Kisspeptin is likely to be one of the factors that induce TSCC to metastasis.2,The tumour in lymph node metastasis with high expression of CD44v3 is likely to have more powerful ability of anti-adhere in TSCC.3,No mutual interaction is found between Kisspeptin and CD44v3 in the metastasis in TSCC.