Studies Of Injuries And Protection Of The Intestinal Barrier In Rats With Acute Hepatic Failure

AIM 1.To study the injures of intestinal mucosa barrier and biological barrier in acute liver failure(ALF),as well as the translocation of bacteria and endotoxemia caused by it. 2.To observe the protection of microecologics on intestinal barrier in ALF. METHODS 66 male Wistar rats were divided into five groups randomly: 10 normal rats served as control group; the rest rats were divided into ALF model group(14 rats), therapy control group(14 rats) prebiotics therapy group(14 rats) and probiotics therapy group(14 rats). The model of ALF were established in the last four groups by subcutaneous injection of thioacetamide(TAA) for consecutive 2 days . The prebiotics therapy group were feed lactulose and the probiotics therapy group were feed bifidobacterium mixture simultaneously. All rats were killed on the third day.The blood endotoxin and ALT was detected; the mesenteric lymph nodes, tissue of spleen and liver ,and the content of intestinal were collected and cultivated to detect bacterial translocation and count intestinal aerobe; the liver and ileal tissue were collected for histological studies, and the liver tissue necrosis area and the intestinal villus height were further measured by the computer-assisted image processing system. RESULTS The pathologic lesions were found in the intestinal mucosa and the liver tissue of the model group and the intestinal villus height shorten significantly Compared with the normal control group; the intestinal aerobe count was rised and the bacterial translocatijon and endotoxemia was observed. All markers of the two therapy groups were ameliorated compared with the therapy control group. CONCLUSION The intestnal mucosa barrier and biological barrier were damaged in rats with ALF and bacterial translocation and endotoxemia can be observed. Microecologics was effective for the prevention and therapy of intestinal barrier injures in ALF.