On The Spleen And The Dampness Party Taa Induced Mouse Model Of Acute Liver Failure

Objective:At present, the mortality of acute liver failure is still particularly high (>80%) in China, mainly because lack of effective drugs. The model that TAA induced acute liver failure is frequently research hepatic encephalopathy and most studies using TAA is performed in rats.However,the mouse model that TAA induced acute liver failure by single injection has the characteristics of high repetition rate,short cycle,but the best dose of TAA to induce acute liver failure with injury of intestine in mice is still not well established.ALF falls into the "yellow jaundice or fulminant jaundice" category on the basis of theory of Traditional Chinese Medicine (TCM), and we have a rich history in treating with it using Chinese herbs. Therefore, to provide more strategies against high mortality of ALF, the author aims to screen effective and target-definite herbs through murine model based on the theory of TCM and clinical practice.Methods:1.We used to mouse model that TAA induced acute liver failure to screen optimum mouse lethal dose via observing96hours survival rate and liver pathology with H.E staining proceedes to evluate.2.We used to mouse model that TAA induced acute liver failure to screen optimum mouse not-lethal model dose via observing24hours survival rate and liver pathology with H.E staining proceedes to evluate.3.we intend to screen effective formula that can alleviate ALF from Xi jiao di huang Decoction(XJDH),Huang lian jie du Decoction, Si jun zi Decoction (ZJZ),Yin chen hao Decoction(YCH) and Jian pi hua shi jie du Decoction (JPHSJD) through TAA-induced lethal murine model, and evaluate the curative effect by observing96hours survival rate.Results:1.200mg/kg is the best model lethal dose that TAA induced acute liver failure murine model,and with obvious intestinal injury.Many experiments:â‘ single intraperitoneal injection of200mg//kg TAA induced acute hepatic failure model survival rate is10%-20%.â‘¡liver pathology with H.E. staining showed that:200mg//kgTAA lethal dose model can induced acute liver injury that showed liver cell infiltration and necrosis,and injury of the small intestine.2.50mg/kg is the best model not-lethal dose that TAA induced acute liver failure murine model,and with intestinal injury.Many experiments:â‘ single intraperitoneal injection of50mg//kg TAA induced acute hepatic failure model survival rate is100%.â‘¡liver pathology with H.E.staining showed that: 50mg//kgTAA not-lethal dose model can induced liver injury and damage of the small intestine.3. TAA can cause multiple organ damageIntrestingly,TAA induced acute liver failure non-lethal model found renal injury. We believe that, TAA can induce multiple organ injury in a some extent.4. Jianpi Huashi Jiedu Decoction can significantly improve the survival rate of mice with acute liver failure.many experiments:Jianpi Huashi Jiedu Decoction can improve the survival rate to approximately40%-50%mice.5. Jianpi Huashi Jiedu Decoction can not reduce the serum transaminase levels in mice with acute liver failure.Serum transaminase levels of Jianpi Huashi Jiedu Decoction has no decline. We suggest that Jianpi Huashi Jie du Decoction could improve survival rate via repairing the other organs.Conclusion:1.200mg/kg is the best model lethal dose that TAA induced acute liver failure murine model.2.50mg/kg is the best model not-lethal dose that TAA induced acute liver failure murine model.3.TAA-induced murine model of acute liver failure can cause multiple organ damage in some extent.4. Jianpi Huashi Jie du Decoction to improve the survival rate of mice.5. Jianpi Huashi Jie du Decoction to improve the survival rate of mice and repair other organs.