Correlation Between The Expression Of Survivin And VEGF In BTCC And The Clinical Significance

The bladder carcinoma is one of the most common urinary malignancies in China, which has a increasing morbidity in recent years. It' s indicated the male mortality rate is about 4.2/100,000 per year and the female mortality rate is 1. 1/100, 000. The majority of pathologic type bladder carcinoma is bladder transitional cell carcinoma (BTCC) which accounts for about 92.8%. The therapy for BTCC is less efective just because of the early relapse. A lot of investigations had been carried out to determine the molecularly biological mechanisms which contributed to the progression of BTCC. It' s suggested the dysregulation of proliferation and apoptosis was the main reason for the malignant transformation and tumor progression. Survivin, one new member of inhibitor of apoptosis protein, being identified recently, can block the apoptosis downstream by inhibiting caspase-3 and -7. Survivin is undetectable in normal adult tissues, meanwhile it' s abundantly expressed in many common human malignancies. To a great extent, it' s provedthat vascular endothelial growth factor (VEGF), play a crucial role in the process of tumor invasion and metastasis. VEGF is the most important factor in regulating angiegenesis of tumor. Up to now, to our knowledge, the study on the expression of surviving and VEGF, and the relationship of co-expressionamong them in BTCC tissues has not yet reported in world. At the same time, there is little information on the clinical pathologic significances on the expression of survivin and VEGF in BTCC.Objective: In this study, the expression of survivin and VEGF was detected by in situ hybridization assay in the BTCC tissues, corresponding none tumorous bladder and non-cancerous bladder tissues. And the relationship between the expression and the clinical pathologic variables and the possible co-expression among them were evaluated.Materials and Methods: 42 cases BTCC that include 30 male patients and 12 female patients and 20 cases corresponding adjacent nontumorous bladder tissues surgically resected from 2003. 8 to 2004. 4, fixed in 1% polyformalin and embedded in OCT were collected. 16 cases non-cancerous bladder tissues resected for bladder disease were arranged as the controls. Expression of surviving and VEGF were detected by in situ hybridization and SABC method. The percentage of positivestained cells were calculated. All the data were processed by the SPSS 10.0 for windows statistically.Results: Of the 23 (54. 8 %) cases of BTCC expressing survivin mRNA in 42 cases of BTCC , 21 were positive and 2 were negative for the expression of VEGF mRNA. Of the 19 cases not expressing survivin mRNA , 1 case was positive and 18 cases were negative for VEGF mRNA. Survivin mRNA and VEGF mRNA was not detected in non-cancerous adjacent bladder tissues and nontumorou bladder tissues. There were significant diferences when the positive rate in BTCC group was compared with that in adjacent group and nontumorous group(P<0.05). There was a close correlation between the expression of survivin and VEGF , proved by the correlation test. There was significant correlation between the expression of survivin mRNA and VEGF mRNA in BTCC and the tumor differentiated level , clinical stages and replase of BTCC. There was no correlation between the expression of survivin mRNA and VEGF mRNA in BTCC and the age and the sex of the patients.Conclusions: survivin and VEGF are over-expressed in BTCC tissues, which have higher positive rates than those of adjacent non-cancerous tissues and nontumorous bladder tissues.It means the progression of BTCC is related possiblyto the up regulation of expression of survivin and VEGF, to some extent. The over expression of survivin and VEGF have a close correlation to the clinicopathologic variables. The detection of survivin and VEGF can be of value in assessing the prognosis for the patients with BTCC.